Assessment the Antibacterial Properties of the Gold Nanoparticles Produced by Fusarium Oxysporum, Conjugated Streptomycin

Authors
Nasrin Naimi Shamel
Samaneh Dolatabadi
Parastoo Pourali
Abstract
Introduction and Aims

The development of eco-friendly methods for the synthesis of nanomaterial shape and size is an important field of nanotechnology. In the present study extracellular biosynthesis of gold nanoparticle was done by Fusarium oxysporum fungus strain and the obtained nanoparticles were conjugated with streptomycin. Finally, the anti-bacterial property of the conjugated gold nanoparticles, was studied.

Materials and Methods

Fusarium oxysporum cultured in Sabouraud Dextrose Broth (SDB) medium. Culture supernatant was subjected to chloroauric acid solution at the final concentration of 1 mmolar. Mixture was used to confirm the presence of nanoparticles with spectrophotometry methods, X-ray diffraction analysis (XRD) and Transition Electron Microscope (TEM). Conjugation of the antibiotics with gold nanoparticles was confirmed through spectrophotometry methods, TEM and infrared spectroscopy (FTIR). Minimal Inhibitory Concentration (MICs) of the mixtures evaluated by broth dilution methods and agar well diffusion plate.

Results

Gold nanoparticles had spectral absorption peak around 530 nm wavelength. XRD results confirmed presence of the elemental gold in the mixture and TEM images have shown the nanoparticles were spherical or hexagonal and their sizes were around 25-30 nanometer. Spectrophotometry, TEM and FTIR results confirmed the conjugation process. Based on the structure of the used antibiotics and the tested bacterial strains, gold nanoparticles that were conjugated with the antibiotics had different antibacterial properties.

Conclusion

Biological production of gold nanoparticles by Fusarium oxysporum can be done and conjugation of gold nanoparticles with streptomycin confirmed without any additional linkers.



* Corresponding Author: Neyshabur Branch, Islamic Azad University, Department of Biology.

Email: S_dolatabadi1436@yahoo.com
Keywords
biosynthesis
metal nanoparticles
streptomycine
Gericke M, Pinches A. Microbial production of gold nanoparticles. Gold Bulletin, 2006;39(1):22-8.

Mollazadeh Moghaddam K. An introduction to microbial metal nanoparticle preparation method. 2010;19(19):1-6. [Persian]

Zhang X, Yan S, Tyagi R, Surampalli RY. Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere. 2011;82(4):489-94.

Sajjadi G, Shojaiee A, Fazeli MR, Amini J, Jamalifar H. Study of ability Fusarium Oxysporum in extracellular biosynthesis of silver nanoparticle in vitro. Journal of Microbial World, 2009;2(1):44-7. [Persian]

Hosseini MR, Schaffie M. Kinetic study of the biological copper removal from a copper sulfate solution in the form of copper sulfide nanoparticles. Journal of separation science and engineering, 2013;4(2):75-82. [Persian]

Donaldson K, Tran L, Jimenez LA, Duffin R, Newby DE, Mills N, et al. Combustion-derived nanoparticles: a review of their toxicology following inhalation exposure. Part Fibre Toxicol. 2005;2:10.

Cui Y, Zhao Y, Tian Y, Zhang W, Lu X, Jiang X. The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli. Biomaterials. 2012;33(7):2327-33.

Castro-Longoria E, Vilchis-Nestor AR, Avalos-Borja M. Biosynthesis of silver, gold and bimetallic nanoparticles using the filamentous fungus Neurospora crassa. Colloids Surf B Biointerfaces. 2011;83(1):42-8.

Klancnik A, Piskernik S, Jersek B, Mozina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods. 2010;81(2):121-6.

Rastogi L, Kora AJ, Arunachalam J. Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics. Mater Sci Eng C Mater Biol Appl. 2012;32(6):1571-7.

Rautaray D, Sanyal A, Adyanthaya SD, Ahmad A, Sastry M. Biological synthesis of strontium carbonate crystals using the fungus Fusarium oxysporum. Langmuir. 2004;20(16):6827-33.

Kittel C. Introduction to Solid State Physics. Wiley; 2004.

Kathiresan K, Manivannan S, Nabeel M, Dhivya B. Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surf B Biointerfaces. 2009;71(1):133-7.

Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci. 2003;85(2):162-70.

Holmes JD, Smith PR, Evans-Gowing R, Richardson DJ, Russell DA, Sodeau JR. Energy-dispersive X-ray analysis of the extracellular cadmiumsulfide crystallites of Klebsiella aerogenes. Arch Microbiol. 1995;163(2):143-7.

Bhattacharya D, Saha B, Mukherjee A, Santra CR, Karmakar P. Gold nanoparticles conjugated antibiotics: stability and functional evaluation. J Nanosci Nanotechno. 2012;2(2):14-21.