Histomorphometric Study of Examination of the Aqueous Extract Effect of Grape (Vitis Vinifera) on Wound Healing Diabetic Male Wistar Rats

Authors
Haleh Lolaavar
Abdolhossein Shiravi
Vida Hojati
Sahar Molzemi
Abstract
Introduction and Aims

In the event of damage to the skin, the epidermis of which goes to the disintegration of the body, is a wound that is common in diabetics. The purpose of this study was to evaluate the effect of aqueous extract of grape (Vitis Vinifera) on wound healing in diabetic Wistar rat.

Materials and Methods

In this study 48 male Wistar rats were divided into 4 groups (control, sham, experimental 1, empirical 2) were tested. The extra pawn injured area of 3 cm to the left of the spine created. Extract-treated group compared to one in three (aqueous extract + eucerin) received. Wound healing was examined microscopically during 21 days.

Results

Wound healing in group that became diabetes using streptozocin (the diabetic rats), showed late recovery compared to normal wound healing in normal groups and wound healing in experimental groups treated with aqueous extract of grape was accelerated compared control group.

Conclusion

The results showed that the aqueous extract of grape skin accelerate wound healing in normal and diabetic samples.



*Corresponding Author: Islamic Azad University Damghan Branch, Faculty of Sciences.

Email: Shiravi738@yahoo.com
Keywords
wound healing
extract
grape seed
Rats
Diabetes
Mordes JP, Bortell R, Blankenhorn EP, Rossini AA, Greiner DL. Rat models of type 1 diabetes: genetics, environment, and autoimmunity. ILAR J. 2004;45(3):278-91.

McGrath JA, Breathnach SM. Wound healing. In: Breathnach SM. Textbook of dermatology. 7th ed. New York: Blackwell; 2004: 1-11.

Desiree MO, Tania JPh. Sex Hormones and Wound Healing. Wounds. 2006;18(1):8-18.

Kaneto H, Katakami N, Kawamori D, Miyatsuka T, Sakamoto K, Matsuoka TA, et al. Involvement of oxidative stress in the pathogenesis of diabetes. Antioxide Redox Signal. 2007;9(3):355-66.

Sher H. Ethnoecological evaluation of somemedicinal and aromatic plants of Kot Malakand Agency, Pakistan. Sci Res Essays. 2011;6(10):2164-73.

Isah AB, Ibrahim YK, Abdulrahman EM, Ibrahim MA. The hypoglycaemic activity of the aqueous extract of Stachytarpheta angustifolia (Verbanaceae) in normoglycaemic and alloxaninduced diabetic rats. Pak J Biol Sci. 2007;10(1):137-41.

Wandell PE. Quality of 1ife of patients with diabetes mellitus. An overview of research in primary health care in the Nordic countries. Scand J Prim Health Care. 2005;23(2):68-74.

Riaz M, Ahmad M, Rahman N. Antimicrobial screening of fruit, leaves, root and stem of Rubus fruticosus. J Med Plant Res 2011;5(24):5920-4.

Alah gahi F,shiravi A, Hojati V. Effect of Alcoholic extract of Grape (VITIS VINIFERA) on wound healing diabetic mail wistar rats. Iranian journal of Diabetes and Metabolism, 2016;15(3):177-82. [Persian]

Wang WD, Xu ShY. Degradation kinetics of anthocyanins in blackberry juice and concentrate. J Food Eng. 2007;82(3):271-5.

Verma R, Gangrade T, Punasiya R, Ghulaxe C. Rubus fruticosus (blackberry) use as an herbal medicine. Pharmacogn Rev. 2014;8(16):101-4.

Lee WC, Kim JK, Kang JW, Oh WY, Jung JY, Kim YS, et al. Palmatine attenuates D-galactosamine /lipopolysaccharide-induced fulminant hepatic failure in mice. Food Chem Toxicol. 2010;48(1):222-8.

Foruotan B, Molzemi S, Haratipor H, Molzemi Sh, Bolbol haghighi N, Alam-Alhoda F, et al. Evaluation of palmatine effects on cutaneous wound healing in normal and diabetic rats. Iranian journal of diabetic and metabolism, 2014;13(5):393-9. [Persian]

Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol. 2009;123(1):22-6.