Effect of Aqueous Extract of Ferula Assa-Foetida and Avicennia Marina on Oxidative Stress Parameters of Brian Cells in Diabetic Rats

Authors
Raheleh Rahbarian
Heshmat Sepehri Moghadam
Seyed Damun Sadoughi
Abstract
Introduction

Increased insulin resistance, inflammatory factors, and oxidative stress parameters are associated with the development of diabetes complications. The aim of this study was to evaluate the effect of Ferula assa-foetida and Avicennia marina aqueous extracts on the activity of antioxidant enzymes of brain cells in diabetic rats.

Materials and Methods

In this experimental study, 54 male rats were randomly divided into the equal groups of control, diabetic control and experimental diabetic 1 and 2. Experimental diabetic and diabetic control groups became diabetes by an intraperitoneal injection of 120 mg/kg alloxan. The experimental diabetic groups received the aqueous extract of Ferula assa-foetida (100 and 200 mg/kg, ip) and Avicennia marina (100 and 200 mg/kg, ip) in alternate days for one month. Sterile distilled water was injected to the control and diabetic control groups. On 1st, 15th and 30th day after induction of experimental diabetes, the levels of superoxide dismutase (SOD), glutathione s-transferase (GST), catalase (CAT) antioxidant enzymes and malondialdehyde (MDA) level were measured in the brian cells. Data analyzed by two way repeated measures ANOVA and post-hoc LSD test.

Results

Injection of Ferula assa-foetida 200 mg/kg, ip) and Avicennia marina extracts 200 mg/kg, ip) to the diabetic rats resulted in a significant decrease in the MDA levels and significant increases in the level of SOD, GST, CAT enzymes in brian cells (P<0.05).

Conclusion

The results of this study confirms the dose-dependent antioxidant role of Ferula assa-foetida and Avicennia marina extracts on improving the oxidative stress in the brain cells of diabetic rats.
Keywords
Enzymes
Antioxidant
Brian
Diabetes
Rats

Sheikhpour R. Diabets and oxidative stress: The mechanism and action. Ijdo 2013; 5(1):40-5.
Pop-Busui R, Sima A, Stevens M. Diabetic neuropathy and oxidative stress. Diabetes Metab Res Rev 2006; 22(4):257-73.
Hosseini A, Abdollahi M. Diabetic neuropathy and oxidative stress: Therapeutic perspectives. Oxid Med Cell Longe 2013; 2013:1-15.
Fatehi-Hassanabad Z, Chan CB, Furman BL. Reactive oxygen species and endothelial function in diabetes. Eur J Pharmacol 2010; 636(1-3):8-17.
Faghihi N, Mohammadi MT, Salem F. Effect of atrovastatin on hyperglysemia induced brain oxidative stress and neuropaty induced by diabetes. J Birjand Univ Med Sci 2015; 21(1):48-58
Jafari Mg, Zangiabadi N, Tajadini H, Shabani M, Sheibani V, Ghasemi GH, et al. Effect of date adua extract and melatonin on diabetic neuropaty in STZ induced diabetic rat. Hormozgan Med J 2012; 17(4):289-97. [Persian]
Hye-won R, Ji-Na L, Hyung-Rhokim M. Protective mechanism of glucose against alloxan–induced B-cell damage. Exp Mol Med 2000; 32(1):12-7.
Vadim D. Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environ Exper Bot 2015; 109:212-28.
Revathi P, Jayaseelan senthinath T, Thiromalaikolundusuberamanian P, Piabhu N. An overview of antidiabetic profile of mangrove plants. Int J Pharm Pharm Sci 2014; 6(3):1-5.
Fathi Moghaddam H, Mokhtari M, Kamaei A, Ahangarpour A. Effects of Avicennia marina leaves aqueous and hydro alcoholic extract on Streptozotocin induced diabetic male rats. J Rafsanjan Univ Med Sci 2011; 10(4):245-54. [Persian]
Mahera SA, Saifullah SM, Ahmad VU, Mohammad FV. Phytochemical studies on mangrove Avicennia marina. Pak J Bot 2013; 45(6):2093-4.
Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of asafetida (Ferula assa-foetida oleo-gum-resin)-a review. J Ethnopharmacol 2011; 134(1):1-10.
Rahbarian R, Sadooghi SD. Investigating the effects of aqueous extract of asafoetida resin on the serum level of insulin and blood glucose in type 1 diabetic rats. Shahrekord Univ Med Sci J 2014; 16(3):16-21. [Persian]
Sadooghi SD. Effects of aqueous extract of asafoetida resin on wound healing of streptozotocin induced diabetic rats. Q Horizon Med Sci 2013; 19(3):129-35. [Persian]
Sadoughi SD, Chamipa M. Effects of aqueous extract of Holothuria arenicola and low frequency electromagnetic field on serum insulin, glucose and beta-amyloid (Aβ1-42) in diabetic rats. Feyz 2016; 20(1):1-10. [Persian]
Sadoughi SD, Edalatmanesh M. The effect of Curcumin and weak low-frequency electromagnetic fields on thyroid hormones in type 1 male diabetic rats. J Zanjan Univ Med Sci 2017; 25(110):34-45. [Persian]
Babizhayev MA, Strokov IA, Nosikov VV, Savel'yeva EL, Sitnikov VF, Yegorov YE, et al. The role of oxidative stress in diabetic neuropathy: generation of free radical species in the glycation reaction and gene polymorphisms encoding antioxidant enzymes to genetic susceptibility to diabetic neuropathy in population of type i diabetic patients. Cell Biochem Biophys 2015; 71(3):1425-43.
Chen CH, Lu Y, Chen Y, Lee W, Lu CH, Chen Y, et al. Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS. J Proteomics 2012; 75(12):3760-77.
Kuo CW, Shen CJ, Tung YT, Chen HL, Chen YH, Chang WH, et al. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. Life Sci 2015; 135:77-86.
Varsha MKNS, Thiagarajan R, Manikandan R, Dhanasekaran G. Vitamin K alleviates streptozotocin-induced type 1 diabetes by mitigating free radical stress, as well as inhibiting NF-κB activation and iNOS expression in rat pancreas. Nutrition 2014; 31(1):214-22.
Salimnejad R, Jalali M, Nikravesh MR, Fazel AR. Effect of Garlic aqueous extract on markers of oxidative stress in diabetic rats testes. J Rafsanjan Univ Med Sci 2014; 13(4):371-82. [Persian]
Farhadi M, Jameie SB, Hayat P. Study of the antioxidant effects of total flavonoiods extracts of Cuscota Lehmanniana Bunge against on PC12 cells in vitro diabetic model. Razi J Med Sci 2011; 18(90):17-26. [Persian]
Davi G, Falco A, Patrono C. Lipid peroxidation in diabetes mellitus. Antioxid Redox Signal 2005; 7(1-2):256-68.
Chang YC, Chuang LM. The role of oxidative stress in the pathogenesis of type 2 diabetes: from molecular mechanism to clinical implication. Am J Transl Res 2010; 2(3):316-31.
Pishgooii A. Diabetic neuropaty and prevention of it. J Army Univ Med Sci IR Iran. 2011; 11(1):7-12. [Persian]
Shrilatha B, Muralidhara. Occurrence of oxidative impairments, response of antioxidant defences and associated biochemical perturbations in male reproductive milieu in the Streptozotocin diabetic rat. Int J Androl 2007; 30(6):508-18.
Kafilzadeh F, ZEinali Sh, Solhjoo K. Evaluation of antibacterial activity of Avicennia marina young branch andmature leaf extract in Khoor-e- TiabHormozgan province. Environ Sci Technol 2015; 17(3):15-23. [Persian]
Behnam-Rassouli M, Ghayour N, Ghayour M, Ejtehadi M. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Med Univ Sci J 2012; 14(6):48-56. [Persian]