The effect of combined training on lipid profile of white blood cells ABCB4 gene expression and HDL to LDL ratio in middle- aged men after coronary artery bypass graft

Authors
Robabe Mahmoodi 1
Amir Rashidlamir 2
Rambod Khajeie 1
Ameneh Barjaste Yazdi 1
1 Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Introduction

Cardiovascular diseases are very common all over the world, including Iran. Meanwhile, atherosclerosis is the leading cause of death worldwide. The aim of this study was to investigate the effect of combined training on ABCB4 gene expression and HDL to LDL ratio in middle-aged men after coronary artery bypass graft.

Materials and Methods

The statistical population consisted 30 middle-aged men that had previously undergone coronary artery bypass graft. This research is quasi-experimental which by random sampling method, they were randomly divided into two groups of control (n=15) and combined training (endurance and resistance, n=15). Blood samples were taken from the two groups before and after the training session. After isolation mononuclear cells by using follicles and mRNA purification, gene expression variation was performed by Real- Time PCR. Data were analyzed by repeated measures variance analysis with SPSS software version 16.

Results

Eight weeks of training resulted in a significant increase in ABCB4 gene expression and significant reduction in plasma total cholesterol levels in the combined training group compared to the control group.

Eight weeks of training resulted in a significant increase in ABCB4 gene expression and significant reduction in plasma total cholesterol levels in the combined training group compared to the control group.

Conclusion

Combined training, as part of cardiac rehabilitation in patients performing coronary artery bypass graft by effecting on ABCB4 gene expression, involved in fat metabolism, improves reverse cholesterol transfer process.
Keywords
Coronary artery bypass graft
Combined training
ABCB4 gene
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