The Effect of Eight Weeks of Moderate-Intensity Interval Training and Vitamin D Intake on Caspase 3 and Caspase 8 Gene Expression in Male Desert Rats Following Induction of Cardiac Infarction

Authors
faezeh vahidmoghadam
maghsoud peeri
shima ababzadeh
forouzan fattahi masror
Abstract
Introduction: The aim of this study was to investigate the effect of an eight-week moderate intensity interval training program and vitamin D intake on caspase 3 and caspase 8 in male desert mice following induction of cardiac infarction.

Methods: Research samples were 56 Wistar male desert rats, which were randomly divided into 7 groups: a control and healthy group, an infarcted Group+5 minutes walking, an infracted group + vitamin D+5 minutes walking, an infracted group +interval training, an infracted group + interval training + vitamin D, an infracted group + interval training + oral paraffin vehicle, and an infracted group + oral paraffin vehicle + 5 minutes of walking. The mice received supplementation, a placebo, and training in specific groups. For statistical analysis, the one-way analysis of variance test was used with the Tukey post-hoc test.

Results: The results showed that the myocardial infarction caused a significant increase in the relative level of caspase-3 and a significant decrease in the relative level of caspase-8 compared to the control group. Both individual and combined interventions caused a significant decrease in caspase3 and a significant increase in caspase8 compared to the infracted group (p=0.001). There was no significant difference between the supplement-only and training-only groups. But the training+supplemental group showed a greater reduction in caspase3 and a greater increase in caspase 8 compared to the other two groups (p=0.001).

Conclusion: The results of this study show that exercise training and supplementation with vitamin D cause a significant change in apoptosis factors, but the combination of the two interventions has a synergistic effect and works better than the individual application of each intervention.
Keywords
Apoptosis
Caspase 3
Caspase 8
Heart Infarction
interval training
Myocardial Infarction
Vitamin D
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