اثر عصاره های آبی و الکلی ریشه باریجه علیه چند سویه پاتوژن باکتریایی

نویسندگان
چکیده
زمینه و هدف

به دلیل مقاومت روزافزون باکتری ها به آنتی بیوتیک ها، نگرش مجدد و منطقی به گیاهان دارویی مهم به نظر می رسد. در این میان باریجه (Ferula gummosa) در مطالعات گذشته به عنوان یک گیاه دارویی مهم مورد توجه بوده است. هدف از مطالعه حاضر بررسی تأثیر عصاره های آبی و الکلی باریجه روی چند سویه باکتریایی پاتوژن می باشد.

مواد و روش ها

عصاره ها توسط دو حلال آب و الکل به روش سوکسله جدا و سپس به روش های انتشار در دیسک، انتشار در چاهک و رقت در لوله مورد بررسی قرار گرفتند.

یافته ها

بر اساس روش دیسک و چاهک هیچ هاله عدم رشدی برای هیچ یک از سویه ها مشاهده نشد. عصاره اتانولی، فعالیت مهار کنندگی بیشتری نسبت به عصاره های متانولی و آبی از خود نشان داد. بیشترین حساسیت در عصاره متانولی مربوط به استافیلوکوکوس اورئوس دارای حداقل غلظت مهار کنندگی و حداقل غلظت باکتری کشی به ترتیب برابر با 103×6/25 و 104×1/25 بود. بیشترین حساسیت در عصاره اتانولی مربوط به اشریشیاکلی و شیگلا دیسانتریه دارای حداقل غلظت مهارکنندگی و حداقل غلظت باکتری کشی به ترتیب برابر با 103×6/25 و 104×2/5 بود.

نتیجه گیری

باریجه فعالیت ضد میکروبی با طیف وسیعی را از خود نشان داد و می تواند به عنوان یک ماده ضد میکروب بالقوه مورد بررسی و تحقیق بیشتری قرار گیرد.



* نویسنده مسئول : دانشگاه آزاد اسلامی واحد تنکابن.

پست الکترونیک: Mohammadsalehi73@gmail.com
کلیدواژه‌ها

عنوان مقاله English

Effect of Aqueous and Alcoholic Extracts of Roots of Ferula Gummosa Boiss on Growth of Some Bacterial Pathogens

نویسندگان English

Seyed Masood Hashemi Karuie
Mohammad Salehi
Maryam Asghar Heidari
Masood Mobiny
Ayatollah Nasrollahi Omran
چکیده English

Introduction and Aims

Because of the day-increasing resistance of the bacteria to antibiotics, further logical viewpoint to the medicinal plants seems to be important. The Ferula gummosa considered as a great medicinal plant in past studies. The aim of this study was investigation of aqueous and alcoholic extracts of roots of F.gummosa Boiss on growth of some bacterial pathogens.

Materials and Methods

The extracts were prepared by Soxhlet method and were studied by disc diffusion, well diffusion and tube dillution methods.

Results

According to the disc diffusion and the well diffusion methods, no inhibitory zone have seen against investigated bacteria. The ethologic extract showed more inhibitory effect than the methanolic and aquatic extracts. The most sensitive bacterium to methanol extract was Staphylococcus aureus with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) equal to 6.25×103 and 1.25×104 respectively. The most sensitive bacteria to ethanol extract were Escherichia coli and Shigella dysenteriae with MIC and MBC equal to 6.25×103 and 2.5×104 respectively.

Conclusion

Further study is needed about potent antimicrobial activities of F.gummosa Boiss.



* Corresponding Author: Islamic Azad University, Tonekabon Branch.

E-mail: Mohammadsalehi73@gmail.com

کلیدواژه‌ها English

Antimicrobial Activity
Ferula gummosa
Crude extracts
Bacteria
MIC
Powers JH. Antimicrobial drug development–the past, the present, and the future. Clin Microbiol Infect. 2004;10(4):23-31.

Andersson DI, Hughes D. Antibiotic resistance and its cost: is it possible to reverse resistance?. Nat Rev Microbiol. 2010;8(4):260-71.

Levin BR, Rozen DE. Non-inherited antibiotic resistance. Nat Rev Microbiol. 2006;4(7):556-62.

Rios J, Recio M. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005;100(1-2):80-4.

Mørk T, Waage S, Tollersrud T, Kvitle B, Sviland S. Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet Scand. 2007;49(1):23.

Turkyilmaz S, Kaya O. Determination of some Virulence Factors in Staphylococcus Spp Isolated from Various Clinical Samples. Turk J Vet Anim Sci. 2006;30:127-32.

Jay JM, Loessner MJ, Golden DA. Modern food microbiology, 7th ed. New York: Springer Science; 2005,583-90.

Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991;4(1):80-128.

Lafont F, Van Nhieu GT, Hanada K, Sansonetti P, van der Goot FG. Initial steps of Shigella infection depend on the cholesterol/sphingolipid raft‐mediated CD44-IpaB interaction. EMBO J. 2002;21(17):4449-57.

Jamshidi A, Bassami MR, Afshari Nic S. Identification of Salmonella spp. and Salmonella typhimurium by a multiplex PCR-based assay from poultry carcasses in Mashhad-Iran. Iran J Vet Med. 2009;3(1):43-8.

Nosrat S, Sabokbar A, Dezfoolian M, Tabarraie B, Fallah F. Prevalence of Salmonella enteritidis, typhi and typhimurium from food products in Mofid hospital. Pajouhesh Dar Pezeshki. 2012;36(1):43-8. [Persian]

Ebrahimpoor F, Eydizadeh K. Medicinal Plants. 1st ed. Tehran: Payame Noor University; 2009. [Persian]

Zargari A. Medicinal Plants. 8th ed. Tehran: Tehran University Publication; 2002. [Persian]

Mozaffarian V. [Plant Classification, Morphology-Taxonomy]. 1st ed. Tehran: Amirkabir press; 2005. [Persian]

Mohammadzadeh Milani J, Rezaii K, Safari M, Ghanbarzadeh B, Phillips GO. Extraction and physicochemical properties of Barijeh (Ferula galbaniflua) gum. Journal of Food and Bioprocess Engineerin. 2015;1(2):9-20.

Moreira M, Ponce A, Del Valle C, Roura S. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Sci Technol. 2005;38(5):565-70.

Ramezani M, Hosseinzadeh H, Mojtahedi K. Effects of Ferula gummosa Boiss. fractions on morphine dependence in mice. J Ethnopharmacol. 2001;77(1):71-5.

Shahrokhi N. [Quality control methods for raw materials of herbal medicines]. 1st ed. Tehran: Jahad Daneshgahi Publications of Shahid Beheshti University; 1996. [Persian]

Salehi M, Hashemi Karuie S, Nasrolahi Omran A, Mobini M, Asghar Hedari M. Effect of aqueous and alcoholic extracts of roots of Ferula gummosa Boiss. on the growth of Pseudomonas aeruginosa. J Gorgan Uni Med Sci. 2013;15(4):18-22. [Persian]

Salehi M, Hashemi Karoui M, Nasrollahi Omran A, Mobini M, Aaghar Heydari M. Antifungal activity of in vitro aqueous and alcoholic extracts of Barije root (Ferula gummosa). J Birjand Uni Med Sci. 2015;21(4):444-50. [Persian]

CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-Eleventh Edition. 2012.

CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-Tenth Edition. 2015.

National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standards. NCCLS Document M7-A5. National Committee for Clinical Laboratory Standards, Wayne. 2001.

Ghasemi Y, Faridi P, Mehregan I, Mohagheghzadeh A. Ferula gummosa fruits: an aromatic antimicrobial agent. Chem Nat Compd. 2005;41(3):311-4.

Abedi D, Jalali M, Asghari G, Sadeghi N. Composition and antimicrobial activity of oleogumresin of Ferula gumosa Bioss. essential oil using Alamar Blue™. Res Pharm Sci. 2008;3(1):41-5.

Ibraheim ZZ, Abdel‐Mageed WM, Dai H, Guo H, Zhang L, Jaspars M. Antimicrobial antioxidant daucane sesquiterpenes from Ferula hermonis Boiss. Phytother Res. 2012;26(4):579-86.
Hilan C, Sfeir R, El Hage R, Jawich D, Frem ME, Jawhar K. Evaluation of the antibacterial activities of Ferula hermonis (Boiss.). Leban Sci J. 2007;8(2):135-51.

Bashir S, Alam M, Ahmad B, Aman A. Antibacterial, anti-fungal and phytotoxic activities of Ferula narthex Boiss. Pak J Pharm Sci. 2014;27(6):1819-25.

Chaudhry NMA, Tariq P. In vitro antibacterial activities of kalonji, cumin and poppy seed. Pak J Bot. 2008;40(1):461-7.

Ertürk Ö. Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants. Biologia. 2006;61(3):275-8.