Study of the Effect of Gold Nanoparticles on HPG Axis (Hypothalamic-Pituitary-Gonad) in Syrian Male NMRI

Authors
Mahnaz Alikhani
Abdolhossein Shiravi
Vida Hojjati
Abstract
Introduction

Nanoparticles manufactured using nanotechnology, have always been a concern because of their toxic and harmful effects, despite having numerous benefits. In this study, the effect of gold nanoparticles on LH and FSH hormones as well as testosterone was investigated in males NMRI.

Materials and Methods

30 male NMRI were classified randomly into 3 groups of 10 including control and experimental groups where the mouse received 50ppm and 100ppm of nanoparticles. Then, gold nanoparticles were injected for 7 days using intraperitoneal injection and after the seven-day period, animals were anesthetized using ketamine and xylazine and blood samples are taken from heart. Then, they were dissected and testicle tissue was extracted to be prepared to perform histological studies.

Results

In this study, significant differences was observed in concentration of LH and FSH hormones (increased concentration) in taken doses and significant changes were observed in testosterone hormone (decrease) in doses of 100 and 50 ppm that could be because of the inhibiting effect of gold nanoparticles on the performance of testosterone producing cells. Statistical calculations were performed using the statistical software version SPSS v.21.

Conclusion

Results of this study showed that gold nanoparticles change the amount of LH and FSH hormones as well as testosterone in male Syrian mouse and this will result in interference with metabolism system.
Keywords
Nanoparticles
LH and FSH Hormones
Testosterone
Testicle
Syrian Male
Alavi-Shoushtari S, Rezai SA, Ansari M, Khaki A. Effects of the seminal plasma zinc content and catalase activity on the semen quality of water buffalo (Bubalus bubalis) bulls. Pak J Biol Sci 2009; 12(2):134-9.
Sung JH, Ji JH, Park JD, Song MY, Song KS, Ryu HR, et al. Subchronic inhalation toxicity of gold nanoparticles. Part Fibre Toxicol 2011; 8:16.
Lasagna-Reeves C, Gonzalez-Romero D, Barria MA, Olmedo I, Clos A, Sadagopa Ramanujam VM, et al. Bioaccumulation and toxicity of gold nanoparticles after repeated administration in mice. Biochem Biophys Res Commun 2010; 393(4):649-55.
Abdelhalim MA, Jarrar BM. Histological alterations in the liver of rats induced by different gold nanoparticle sizes, doses and exposure duration. J Nanobiotechnology 2012; 10:5.
Warheit DB, Webb TR, Colvin VL, Reed KL, Sayes CM. Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics. Toxicol Sci 2007; 95(1):270-80.
Tomii Y. Lipid formulation as a drug carrier for drug delivery. Curr Pharm Des 2002; 8(6):467-74.
Arora S, Jain J, Rajwade JM, Paknikar KM. Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells. Toxicol Appl Pharmacol 2009; 236(3):310-8.
Park KH, Chhowalla M, Iqbal Z, Sesti F. Single - walled carbon nanotubes are a new class of ion channel blockers. J Biol Chem 2003; 278(50):50212-6.
Bianco A, Wu W, Pastorin G. Carbon nanotube-based vectors for delivering immunotherapeutics and drugs. In: Kumar Challa SSR, ed. Nanomaterials for medical diagnosis and therapy. Nanotechnologies for the life sciences. Weinheim: Wiley-VCH; 2007. p. 85-142.
Chen X, Schluesener HJ. Nanosilver: A nanoproduct in medical application. Toxicol Lett 2008; 176(1): 1-12.
Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR). Opinion on the appropriateness of existing methodologies to assess the potential risks associated with engineered and adventitious products of nanotechnologies. Adopted by the SCENIHR during the 7th plenarymeeting of 28-29. 2005; 5(8):50-9.
Rezaei Zarchi S, Taghavi Foumani MH, Negahdary M. Effect of silver nanoparticles on the hormones LH, FSH and testosterone in male rats. J Babol Univ Med Sci 2013; 15(1):25-9.
Fatahian Dehkordi RA, Heidarnejad S, Ameri A. ZnO nanoparticles effect of on male rat gonad histology and its effect on blood serum sex factors. J Cell & Tissue 2015; 6(2):187-94.