بررسی تاثیر عصاره هیدروالکلی گیاه اسطوخودوس بر عفونت ناشی از کیست ژیاردیا لامبلیا در موش سوری

نویسنده
حسین وزینی
چکیده
مقدمه

ژیاردیا لامبلیا یکی از ‌‌شایع‌‌ترین تک یاخته‌‌های دستگاه گوارش انسان می‌‌باشد. در حال حاضر داروهای ضد ژیاردیایی دارای اثرات جانبی مختلفی می‌‌باشند. هدف از این مطالعه بررسی تاثیر عصاره هیدروالکلی گیاه اسطوخودوس بر روی ژیاردیا لامبلیا می‌‌باشد.

مواد و روش‌‌ها

در مطالعه حاضر تعداد 25 سر موش سوری بطور تصادفی به 5 گروه که عبارتند از: کنترل سالم، کنترل دارو (مترونیدازول)‌‌ و 3 گروه آلوده به انگل تحت درمان با دوزهای 200،100 و mg/ml 400 عصاره اسطوخودوس تقسیم شدند. عصاره اسطوخودوس به حیوانات تأثیر داده شد و پس از گذشت 10 روز، درصد کیست‌‌های زنده محاسبه گردید. روش‌‌های آماری بکار رفته بر مبنی تحلیل واریانس یک طرفه و مقایسه t زوجی بوده که به روش معمول برای مقادیر نرمال و به روش بوت استرپ برای مقادیری که توزیع‌‌شان نرمال نبود با توجه به حجم پایین نمونه استفاده گردید.

یافته‌‌ها

میانگین وزن موش‌‌ها در گروه آلوده به انگل و درمان شده با دوز mg/ml 100 اسطوخودوس در مقایسه با دو گروه آلوده به انگل و درمان شده با دوز 200 و mg/ml 400 اسطوخودوس دارای وزن بیشتری بودند. نتایج حاصل از این مطالعه نشان داده است که کاهش تعداد کیست در گروه‌‌های 100، 200 و mg/ml 400 به ترتیب برابر با 77/7، 84/3 و 95/1 درصد بوده است.

نتیجه‌‌گیری

نتایج حاصل از این مطالعه نشان داده است که گیاه اسطوخودوس در شرایط درون تنی اثرات بسیار خوبی از خود نشان داده است و می‌‌تواند پتانسیل درمانی برای عفونت ناشی از ژیاردیا را داشته باشد.
کلیدواژه‌ها
ژیاردیا لامبلیا
اسطوخودوس
موش کوچک آزمایشگاهی
عفونت

عنوان مقاله English

The Invivo activity of Lavandula angustifolia on Giardia lamblia in souri miceThe Invivo activity of Lavandula angustifolia on Giardia lamblia in souri mice

نویسنده English

Hossein Vazini
چکیده English

Introduction

Giardia lamblia is one of the most prevalent flagellate protozoa in human with the world-wide distribution. Millions of people are affected by Giardiasis annually. Today, many treatments are available for giardiasis that associated with some side effects. This study is aimed to evaluate the antigiardial activity of Lavandula angustifolia in vivo.

Materials and Methods

In this study 25 mice are categorized in 5 groups include; Control group (without any treatment), Positive control (treatment by Metronidazole) and other three groups (treatment by 100, 200 and 400 mg/ml of Plant extracts). Then, after 10 days, the effects of each group are evaluated on the Giardia lamblia. The statistical methods used were based on one-way variance analysis and paired t-comparison that were used in the usual way for normal values in the Bootstrap method for values that were not distributed normalized according to the low sample size.

Results

In this study, the body weights of treated mice with different extracts were more than the positive control and less than the negative control. Also, the live cysts were decreased 77.7, 83.4 and 95.1% by the use of 100, 200 and 400 mg/ml of plant extracts.

Conclusion

The results of the current study indicated that Lavandula angustifolia has conceivable effects in vivo and it will be a suitable alternative for treatment of Giardiasis.

کلیدواژه‌ها English

Giardia lamblia
Lavandula angustifolia
Souri mice
Infection
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