Evaluation of Total Antioxidant and Homocystein levels in the Type II Diabetic Patients

Authors
reyhane rezaei
Gholamreza Anani sarb
mohammad malekaneh
Birjand University of Medical Sciences
Abstract
Introduction: In type 1 and type 2 diabetes, oxidative stress increases in the body. Oxidative stress is an imbalance between the production of free oxygen radicals and the antioxidant defense in the body. Hyperglycemia elevated the level of glucose oxidation in the several ways that leads to more radical production. Levels of some pro-oxidants such as ferritin and homocytine increase in diabetes. The increaed production of free oxygen radicals play an important role in the induction of vascular damages in the diabetic patients.

Materials ad Methods:. The population of this cross-sectional study was the selected diabetic patients referred to Diabetes Clinic of Birjand University of Medical Sciences. Blood samples were collected from 81 patients and 35 healthy subjects. Levels of hemocysteine and total antioxidant serum were measured by EIA and FRAP methods, respectively.

Results: The mean of hemocystein for the neprhropathy patients was 13.8 ± 10.9, retinopathy patients; 11.7 ± 4.9, diabetic paitients;16.6 ± 9.5;control group; 13.2 ± 7.4, p= 0.153. The mean of antioxidant levels in the nephropathy, retinopathy, diabetic, and control groups were 848.9 ± 2.22, 860.8± 150.51, 812.1 ± 171, 986 ± 260 respectively, p= 0.007. The results showed that there is a statistically significant difference between groups.

Conclussion:This study showed that there is no relationship between homocysteine ​​level and type 2 diabetes, but the antioxidant level in type 2 diabetes is reduced.
Keywords
Homocystein
Antioxidants
Diabet
Oxidative stress
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes care 2006; 29(suppl1):43-8.
Pulsinelli WA, Levy DE, Sigsbee B, Scherer P, Plum F. Increased damage after ischemic stroke in patients with hyperglycemia with or without establisheddiabetes mellitus. Am J Med 1983; 74(4):540-4.
Heart Outcomes Prevention Evaluation (HOPE) Study Investigators. Effects of ramipril on cardiovascular and microvascular outcomes in people with diabetes mellitus: results of the HOPE study and MICRO-HOPE substudy. Heart Outcomes Prevention Evaluation Study Investigators. Lancet 2000; 355(9200):253-9.
Reichard P, Nilsson B-Y, Rosenqvist U. The effect of long-term intensified insulin treatment on the development of microvascular complications of diabetes mellitus. N Engl J Med 1993; 329(5):304-9.
Azimi-Nezhad M, Ghayour-Mobarhan M, Parizadeh MR, Safarian M, Esmaeili H, Parizadeh SM, Khodaee G, et al. Prevalence of type 2 diabetes mellitus in Iran and its relationship with gender, urbanisation, education, marital status and occupation. Singapore Med J 2008; 7(49):571-6.
Hasani-Ranjbar S, Larijani B, Abdollahi M. A systematic review of Iranian medicinal plants useful in diabetes mellitus. Arch Med Sci 2008; 4(3):285-92.
Ganguly P, Alan SF. Role of homocysteine in the development of cardiovascular disese. Nutr J 2015; 14:6.
Kontoangelos K, Papageorgiou C, Raptis A, Tsiotra P, Lambadiari V, Papadimitriou G, et al. Homocysteine, cortisol, diabetes mellitus, and psychopathology. J Diabetes Res 2015; 1-10.
Zatalia SR, Sanusi H. The role of antioxidants in the pathophysiology, complications, and management of diabetes mellitus. Acta Med Indones 2013; 45(2):141-7.
RaniAJ, Mythili S. Study on total antioxidant status in relation to oxidative stress in type 2 diabetes mellitus. J Clin Diagn Res 2014; 8(3):108-10.
Rafieian-Kopaei M, Baradaran A, Rafieian M. Oxidative stress and the paradoxical effects of antioxidants. J Res Med Sci 2013; 18(7):629.
Hamdy MM, Mosallam DS, Jamal AM, Rabie WA. Selenium and Vitamin E as antioxidants in chronic hemolytic anemia: Are they deficient? A case-control study in a group of Egyptian children. J Adv Res 2015; 6(6):1071-7.
Zhang P, Xu X, Li X. Cardiovascular diseases: oxidative damage and antioxidant protection. Eur Rev Med Pharmacol Sci 2014; 18(20):3091-6.
Alvares Delfino VD, de Andrade Vianna AC, Mocelin AJ, Barbosa DS, Mise RA, Matsuo T. Folic acidtherapy reduces plasma homocysteine levels and improves plasma antioxidant capacity in hemodialysis patients. Nutrotion 2007; 27(5):242-7.
Aghamohammadi V PGB, Aliasgharzadeh A. Evaluation of the Level of Plasma Homocysteine in Patients with Type 2 Diabetes Mellitus under Metformin Treatment and its Correlation with Serum Total Antioxidant Capacity, Malondialdehyde, Creatinine, Insulin Resistance and Glycemic Control. zumsj 2010; 19(76):1-10. [Persian]
Faulkner MS, Chao WH, Kamath SK, Quinn L, Fritschi C, Maggiore JA, et al. Total homocysteine, Diet, and lipid profiles in type1 and type2 diabetic and nondiabetic adolescents. J Cardiovasc Nurs. 2006; 21(1):47-55.
Mohammadi M, Aminalroaia A, Rezvanian H, Kachoei A, Amini M. Is there any difference in the plasma homocysteine levels of diabetes mellitus type ii patients, impaired glucose tolerance subjects and normal people? JSSU 2005; 13(3):26-30. [Persian]
Al-nozah M A-dN, Bartlett WA, Al- Attas O, Al-Maatouq M, Martin SC. Serum homocysteine concentraton is related to diabetesmellitus, but not to coronary heart disease in Saudi Arabians. Diabetes Obes Metab 2002; 4(2):118-23.
Elias AN, Eng S. Homocysteine concentrations in patients with diabetes mellitus - relationship to microvascular and macrovascular disease. Diabetes Obes Metab 2005; 7(16):117-21.
Coppola A, Astarita C, Oliviero M, Fontana D, Picardi G, Esposito K, et al. Impairment of coronary circulation by acute hperhomocysteinemia in type 2 diabetic patients. Diabetes Care 2004; 27(8):2055-6.
Parvizi M, Shojapur M, Tavakoli J, Nazem H, Fakhrzadeh H, Heshmat R, et al. [The relationship between the polymorphism of MTHFR gene ad Diabet mellitus]. ijdld 2009; 8(3):281-8.[Persian]
Malekira AA, Beyrami M, Rahzani K, Shariatzadeh MA, Eisabadi Bozchelouei A. A survey of some factors related to total antioxidant capacity of saliva. JSSU 2009; 16(4):87-91. [Persian]
Savu O, Ionescu-Tirgoviste C, Atanasiu V, Gaman L, Papacocea R, Stoian I. Increase in total antioxidant capacity of plasma despite high levels of oxidative stress in uncomplicated type 2 diabetes mellitus. J Int Med Res 2012; 40(2):709-16.
Radi S, Abdolsamadi H, Ranjbar A, Rafieian N, Borzoei S, Roshanaei G, et al. Evaluation of Salivary Level of Paraoxonase and Total Antioxidant Capacity in Type II Diabetic Subjects. ajcm 2015; 22(2):114-21.