بررسی اثر 12 هفته تمرین تناوبی بر بیان ژن FOXO1 بافت چربی زیرپوستی و مقاومت انسولین در رت‌‌های دیابتی نوع 2

نویسنده
افضل الماسی
دانشکده تربیت بدنی و علوم ورزشی، واحد اسلامشهر، دانشگاه آزاد اسلامی
چکیده
مقدمه

تحقیقات به نقش احتمالی FOXO1 در پاتوژنز دیابت نوع 2 بواسطه این ادعا که کنترل گلیسیمیک و متابولیسم چربی را متاثر می‌‌کند اشاره نموده‌‌اند. لذا هدف از تحقیق حاضر بررسی تاثیر 12 هفته تمرینات تناوبی شدید بر بیان FOXO1 در بافت چربی زیرپوستی، انسولین سرم، مقاومت انسولین و گلوکز خون در رت‌‌های دیابتی نوع 2 می‌‌باشد.

مواد و روش‌‌ها

در پژوهش بنیادی- تجربی حاضر، 14 سر رت نر ویستار 10 هفته‌‌ای با وزن 20±220 گرم به شیوه تصادفی انتخاب شدند. رت‌‌های دیابتی شده به 2 گروه تناوبی و کنترل تقسیم شدند. تمرینات 12 هفته به صورت 5 جلسه 30 دقیقه‌‌ای در هفته در قالب دویدن روی تریدمیل با تکرارهای یک دقیقه‌‌ای و استراحت فعال 2 دقیقه‌‌ای انجام شد. گروه دیابتی تمرین نکرده در هیچ برنامه تمرینی شرکت داده نشدند. 48 ساعت پس از آخرین جلسه تمرینی، رت‌‌ها بیهوش و کشتار شدند. از آزمون t-test برای تحلیل آماری استفاده شد.

یافته‌‌ها

12 هفته تمرین تناوبی باعث کاهش معنی‌‌دار بیان نسبی FOXO1 در مقایسه با گروه کنترل شد (0/033=P). هر چند از نظر مقاومت به انسولین در بین دو گروه تفاوت معنی‌‌داری وجود نداشت (0/921=P)، اما مقدار گلوکز و انسولین کاهش معنی‌‌داری در اثر 12 هفته تمرین تناوبی داشتند (0/001=P).

نتیجه‌‌گیری

12 هفته تمرین تناوبی شدید علیرغم عدم تغییر در مقاومت انسولین در رت‌‌های دیابتی نوع 2، به کاهش سطوح گلوکز خون، منجر می‌‌شود. این بهبود را می‌‌توان به نوعی به مسیرهای سیگنالینگ وابسته به تغییر در بیان FOXO1 مستقل از مقاومت انسولین نسبت داد.
کلیدواژه‌ها
FOXO1
دیابت نوع 2
مقاومت به انسولین
موش صحرایی

عنوان مقاله English

Investigation effect of 12-week High-Intensity Interval Training FOXO1 Gene Expression of Subcutaneous Adipose Tissue and Insulin Resistance in Type 2 Diabetic Rats

نویسنده English

afzal almasi
چکیده English

Background and Objectives: Studies have pointed to the possible role of FOXO1 in the pathogenesis of type 2 diabetes, due to the claim that it affects glycemic control and lipid metabolism. The aim of this study was to investigate the effect of 12 weeks of high-intensity interval (HIIT) on FOXO1 expression in subcutaneous adipose tissue, insulin resistance, insulin resistance, and blood glucose in type 2 diabetic rats.

Materials and Methods: This is a fundamental and experimental research. The statistical population of this study is all male Wistar rats of Pasteur Institute of Iran. Among them, 14 male Wistar 10 week old rats weighing 220 ± 20 gr Randomized method was used to participate in the study. After a fasting night (12 hours), rats were diabetic by injection of nicotinamide and streptozotocin and divided into 2 groups of HIIT training and control. 12-week exercises were performed in 5 sessions of 30 minutes per week in the running of the treadmill with 1-minute repetitions and 2-minute active rest. The untrained diabetic group did not attend any exercise program. 48 hours after the last training session, the rats were anesthetized and slaughtered. A t-test was used for statistical analysis.

Results: 12 weeks of periodic exercise decreased the relative expression of FOXO1 in comparison with the control group (P = 0.033). However, there was no significant difference in insulin resistance between the two groups (P = 0.921), but glucose and insulin decreased significantly with 12 weeks of periodic exercise (P = 0.001).

Conclusion: 12 weeks of HIIT training leads to a decrease in blood glucose levels, despite the lack of insulin resistance in type 2 diabetic rats. This improvement can be attributed somewhat to signaling pathways dependent on changes in the expression of FOXO1 independent of insulin resistance.

کلیدواژه‌ها English

FOXO1
Type 2 Diabetes
Insulin Resistance
Rats
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