Effect of Eight-week aerobic training combined with Green Coffee consumption on ABCG8 gene expression, serum Leptin and HDL levels of overweight women

Authors
Najme Sabah Alvizade
Artemis Naghibzade
Samane Drodi
Mojtaba Hoseni
Abolghasem Sakhi
Amir Rashid Lamir
Abstract
Introduction: Heart disease has a direct relationship with the increase of LDL-c and decrease of HDL-c, and 8ABCG has a positive effect on these two variables by preventing atherosclerosis by interfering with the reverse transmission of cholesterol. Therefore, this study aimed to compare the effect of eight weeks aerobic training with green coffee consumption on ABCG8, leptin, and HDL gene expression in overweight women.

Methodology: 30 overweight young women were randomly divided into 10 groups and eight weeks of aerobic training with a frequency of three sessions per week, the intensity of 50% of the maximum heart rate, and in the last two weeks severely increased 75% of the maximum heart rate. one hour before From a practice in a one-way blind, group “Practice and green coffee” received one 400 mg capsule and group “Placebo and green coffee” received Placebo. In order to compare the variations between groups was used one-way ANOVA (P <0.05).

Results: The results showed that the values ​​of all three variables ABCG8, leptin, and HDL-C had significant changes (p> 0.05). The LSD post hoc test in all three variables indicates a significant change between “Practice and green coffee” with Other groups.

Conclusion: Exercise and green coffee can affect the health of people by expression of ABCG8, an increase of HDL-C, a decrease of leptin and weight. Therefore obese people can use this type of exercise and complementary green coffee to prevent cardiovascular disease and weight loss.
Keywords
ABCG8
HDL-c
leptin
green coffee
Alipour Z, Moghadasi M. Effect of eight weeks aerobic training on renal function and cardiovascular risk factors in renal transplant recipients’ women. Journal of Applied Exercise Physiology 2018; 13(26):105-14. [Persian]
Rahmati Ahmadabad S, Shirvani H, Sobhani V. The long-term effects of intense periodic exercises and supplementation of flax seed oil on expression of genes involved in reverse cholesterol transmission in male rats. JMP 2018;4(64):59-75. [Persion]
Shipp LE, Hamdoun A. ATP-Binding Cassette (ABC) Transporter Expression and Localization in Sea Urchin Development. Dev Dyn 2012;241(6):1111-24.
Woodward OM, Kottgen A, Kottgen M. ABCG transporters and disease. FEBS J 2011;278(18):3215-25.
Wang N, Ranalletta M, Matsuura F, Peng F, Tall AR. LXR-Induced Redistribution of ABCG1 to Plasma Membrane in Macrophages Enhances Cholesterol Mass Efflux to HDL. Arterioscler Thromb Vasc Biol 2006;26(6):1310-6.
Haghighi A, Hamedinia MR. Effect of 13 weeks aerobic exercise on serom leptin of obes women. Olympic J 2008;16(1):89-98. [Persion]
Sprung CL1, Annane D, Keh D, Moreno R, Singer M, Freivogel K, et al. Hydrocortisone therapy for patients with septic shock. N Engl J Med 2008;358:111-24.
Bouassida A, Zalleg D, Bouassida S, Zaouali M, Feki Y, Zbidi A, et al. Leptin, its implication in physical exercise and training: a short review. J Sports Sci Med 2006;5(2):172-81.
Boudou P, Sobngwi E, Mauvais Jarvis F, Vexiau P, Gautier JF. Absence of exercise-induced variations in adiponectin levels despite decreased abdominal adiposity and improved insulin sensitivity in type 2 diabetic men. Eur J Endocrin 2003;149(5):421-4.
Chung B, Matak P, McKie AT, Sharp P. Leptin increases the expression of the iron regulatory hormone hepcidin in huh7 human hepatoma cells. J Nutr 2007;137(11):2366-70.
Houshmand Moghadam B, Shinkhiz F. Combined effect of creeping exercises and supplementation of cumin on anthropometric indices, body composition, metabolic, antioxidant and inflammatory in overweight men: A randomized, controlled clinical trial. mjms 2018; 61(2):900-10. [Persion]
Murase T, Misawa K, Minegishi Y, Aoki M, Ominami H, Suzuki Y, et al. Coffee polyphenols suppress diet-induced body fat accumulation by downregulating SREBP-1c and related molecules in C57BL/6J mice. Am J Physiol Endocrinol Metab 2011;300(1):122-33.
Troiano RP, Berrigan D, Dodd KW, Masse LC, Tilert T, McDowell M. Physical activity in the United States measured by accelerometer. Med Sci Sports Exerc 2008;40(1):181-8
Shimoda H, Seki E, Aitani M. Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complement Altern Med 2006;6:9
Dray C, Daviaud D, Guigné C, Valet P, Castan-Laurell I. Caffeine reduces TNFalpha up-regulation in human adipose tissue primary culture. J Physiol Biochem 2007;63(4):329-36.
Haskó G, Cronstein B. Methylxanthines and inflammatory cells. Handb Exp Pharmacol 2011;(200):457-68.
Abe-Dohmae S, Ikeda Y, Matsuo M, Hayashi M, Okuhira K, Ueda K, et al. Human ABCA7 supports apolipoprotein-mediated release of cellular cholesterol and phospholipid to generate high density lipoprotein. J Biol Chem 2004;279(1): 604-11.
Naderi L, Sharifi G. Comparison of the effect of 8 weeks concurrent training and green coffee supplementation on serum adipsin and insulin resistance in obese women. Armaghane danesh 2017;22(5):623-36. [Persion]
Ghanbari–Niaki A, Saghebjoo M, Hedayati M. A Single Session of Ciruit–resistance exercise effects on human peripheral blood lymphocyte ABCA1 expression and plasma HDL–C level. Regul Pept 2011;166(1-3):42-7.
Ramezani Z, Hejazi SM, Rashidlamir A. The effect of eight weeks aerobic exercise on the atherogenic ratio and ABCG8 gene expression in PBMC globules of overweight women. IJDO 2017; 9(3):95-100
Ghanbari-Niaki A. Treadmill exercise training enhances ATPbinding cassette protein-A1 (ABCA1) expression in male rats’ heart and gastrocnemius muscles. IJEM 2010;8(4):206-10.
Faramarzi M, Mousavi Ghahfarokhi M, Khosravi N. [Effect of aerobic training on the fluctuation of pressure and rhythmic low CRP levels in older women]. Sport Physiology 2011;3(10):103-13. [Persion]
Baldan A, Bojanic D, Edwards PA. The ABCs of sterol transport. J Lipid Res 2009;50:80-5.
Ghanbari-Niaki A, Rahmati-Ahmadabad S, Zare-Kookandeh N. ABCG8 gene responses to 8 weeks treadmill running with or without Pistachia atlantica (Baneh) Extraction in Female Rats. Int J Endocrinol Metab 2012;10(4):604-10.
Meissner M, Nijstad N, Kuipers F, Tietge UJF. Voluntary exercise increases cholesterol efflux but not macrophage reverse cholesterol transport in vivo in mice. Nutr Metab 2010;7:54.
Sabeva NS. Regulation of abcg5 and abcg8 sterol trasporters in biliary cholesterol elimination, reverse cholesterol trasport and dyslipidemia [dissertation]. University of Kentucky; 2011.
Mohammadi A, Mirzaei F, Nabi Moradi M, Jamshidi M, Yari R, Ghiasvand T, et al. Effect of flaxseed on serum lipid profile and expression of NPC1L1, ABCG5 and ABCG8 genes in the intestine of diabetic rat. Avicenna J Med Biochem 2013;1(1):1-6.
Ghorbanian B, Ghasemnian A. The effects of interval combined endurance training on some key reverse cholesterol transport factors in boy adolescents. J Urmia Univ Med Sci 2015; 26(3):227-36. [Persion]
Durstine JL, Grandjean PW, Davis PG, Ferguson MA, Alderson NL, DuBose KD. Blood lipid and lipoprotein adaptations to exercise: a quantitative analysis. Sports Med 2001;31(15):1033-62.
Wood PD, Stefanick ML, Williams PT, Haskell WL. The effects of plasma ipoproteins of a prudent weight-reducing diet, with or without exercise, in overweight men and women. N Engl J Med 1991;325(7):461-6
Ghahramanlo E, Agha alinezhad H, Ghara khanlo R. [Comparison of three kinds of strength training, endurence and parallel features bioenergy, maximal strength and body omposition untrained men]. Olympic 2007;13(40):45-58. [Persion]
Gökbel H, Baltaci AK, Üçok K, Okudan N, Moĝulkoç R. Changes in serum leptin levels in strenuous exercise and its relation to zinc deficiency in rats. Biological Trace Element Research 2005;106(3):247-52.
Okazaki T, Himeno H, Nanri H, Ogata M, Ikeda M. Effects of mild aerobic exercise and a mild hypocaloric diet on plasma leptin in sedentary women. Clin Exp Pharmacol Physiol 1999;26(5-6):415-20.
Rodriguez de Sotillo DV, Hadley M: Chlorogenic acid modifies plasma and liver concentrations of cholesterol, triacylglycerol, and minerals in (fa/fa) Zucker rats. J Nutr Biochem 200213:717-26.