اثر درمانی ترکیبی با رزوراترول، ورزشهای تناوبی و تداومی بر وضعیت استرس اکسیداتیو و التهاب در بافت قلبی موش های نر مبتلا به کبدچرب غیرالکلی

نویسندگان
حسین مهدیان 1
پروین فرزانگی 2
امین فرزانه حصاری 2
1 دانشگاه آزاد اسلامی
2 دانشگاه آزاد ساری
چکیده
مقدمه

کبد چرب غیرالکلی (NAFLD) مهمترین اختلال مزمن کبدی می‌باشد. در این مطالعه تاثیر درمان ترکیبی با مکمل رزوراترول و تمرین ورزشی بر روی استرس اکسیداتیو و التهاب در بافت قلبی موش‌های نر NAFLD بررسی شد.

مواد و روش­‌ها

در این مطالعه تجربی، تعداد 56 موش نر به دو گروه NAFLD (49=n) و کنترل (7=n) تقسیم شدند. NAFLD با رژیم غذایی پرچرب در موش­ها القاء و سپس به 7 زیر گروه تجربی بیمار، سالین، رزوراترول (RSV)، ورزش تناوبی، ورزش تداومی، رزوراترول + ورزش تناوبی، و رزوراترول + ورزش تداومی تقسیم شدند. بیومارکرهای استرس اکسیداتیو و التهاب شامل آنزیمهای سوپراکسیددیسموتاز (SOD)، کاتالاز (CAT)، مالون دی آلدئید (MDA)، IL-10 و TNF-α در بافت قلبی موش­ها به روش ELISA اندازه گیری شد. جهت تجزیه و تحلیل داده­ها از روش آماری ANOVA و نرم افزار SPSS استفاده شد.

یافته‌­ها

تجویز رزوراتول به تنهایی و یا همراه با تمرین ورزشی سبب افزایش معنی­داری در میانگین آنزیم های CAT و SOD در بافت قلبی موش­ها در مقایسه با گروه­های بیمار و سالین گردید (p<0.0001)، در حالی­که که سطح MDA کاهش معنی­داری داشت (p<0.05). همچنین رزوراترول به تنهایی و یا در ترکیب با تمرین های ورزشی سبب کاهش معنی­داری در سطح TNF-α و افزایش معنی­داری در غلظت IL-10 شده بود (p<0.001).

نتیجه­‌گیری

کبد چرب غیرالکلی سبب افزایش استرس اکسیداتیو و التهاب سلول­های قلبی می­گردد. اگرچه رزوراترول به تنهایی سبب بهبود استرس اکسیداتیو و التهاب در بافت قلبی گردید، درمان ترکیبی آن با تمرین ورزشی موثرتر می­باشد.
کلیدواژه‌ها
استرس اکسیداتیو
بیماری کبد چرب غیرالکلی
تمرین ورزشی تناوبی و تداومی
رزوراترول
قلب
موش صحرایی

عنوان مقاله English

Effect of combined therapy with resveratrol, continuous and interval exercises on oxidative stress and inflammation in cardiac tissue of male rats with non-alcoholic fatty liver

نویسندگان English

Hossein mahdian 1
parvin farzanegi 2
Amin farzaneh hesari 2
1 Islamic Azad University
2 Islamic Azad University
چکیده English

Background and objective: Non-alcoholic fatty liver (NAFLD) is the most important liver chronic disorder. In this study, combined therapy with resveratrol supplementation alone or in combination with exercise training was considered on oxidative stress status and inflammatory biomarkers in the heart of rats with NAFLD.

Background and objective: Non-alcoholic fatty liver (NAFLD) is the most important liver chronic disorder. In this study, combined therapy with resveratrol supplementation alone or in combination with exercise training was considered on oxidative stress status and inflammatory biomarkers in the heart of rats with NAFLD.

Materials and methods: In this experimental study a total of 56 male rats were divided into two groups, including control (n=7) and NAFLD (n=49). NAFLD was induced using high-fat diets and then the NAFLD rats were randomly divided into seven experimental groups including, patient, saline, resveratrol (RSV), continuous exercise, interval exercise, continuous exercise + RSV, and interval exercise + RSV. Oxidative stress and inflammation biomarkers, including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), IL-10, and TNF-α were measured in the heart tissue using specific ELISA kits. Data were analyzed using ANOVA program by SPSS software.

Results: Resveratrol supplementation alone or in combination with exercise training significantly increased the mean of cardiac CAT and SOD activity compared to the patient and saline groups (p<0.0001), while MDA level was significantly decreased (p<0.05). Furthermore, RSV alone or in combination with exercise training significantly decreased the TNF-α content, but significantly increased the IL-10 level in the heart tissue (p<0.001).

Conclusion: NAFLD is associated with oxidative stress and inflammation in the heart tissue. Although resveratrol alone has an anti-oxidative and anti-inflammatory property, combined therapy with interval and continuous training can be probably more effective.

کلیدواژه‌ها English

Oxidative stress
Non-alcoholic Fatty Liver
Interval and Continuous Trainings
Resveratrol
Heart
wistar Rats
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