اثر محافظتی عصاره آبی سرخ ولیک بعد از دویدن با شدت بالا بر شاخص های آسیب قلبی در موش های صحرایی نر ویستار

نویسندگان
احمد عبدی 1
سیده مریم مصطفویان 1
مریم داوران 1
طاهره حائری 2
1 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران.
چکیده
مقدمه

هدف از پژوهش حاضر بررسی اثر محافظتی عصاره آبی سرخ ولیک بعد از دویدن با شدت بالا بر شاخص­‌های آسیب قلبی در موش‌های صحرایی نر ویستار می‌­باشد.

مواد و روش­‌ها

32 سر موش صحرایی نر ویستار، با وزن 140-173 گرم به طور تصادفی به گروه­‌های کنترل- سالین (SC)، سالین- تمرین (ST)، سرخ ولیک- کنترل (CPC) و سرخ ولیک- تمرین (CPT) تقسیم شدند. گروه­‌های تمرینی به مدت 8 هفته برنامه تمرینی دویدن با شدت بالا (34 متر در دقیقه، 60 دقیقه ، پنج روز هفته) را روی تردمیل انجام دادند. به گروه­‌های CPCو CPT (500 میلی­‌گرم/کیلوگرم) عصاره سرخ ولیک و به گروه­‌های SC و ST به همین مقدار سالین خورانده شد. 72 ساعت پس از آخرین جلسه تمرینی، موش‌­ها کشتار شدند، بافت قلب برداشته و بلافاصله در محلول نیتروژن فریز و در دمای 80- نگهداری شد. پلاسما نیز برای اندازه‌­گیری شاخص­‌های پلاسمایی جمع‌­آوری شد. برای تجزیه و تحلیل آماری از تحلیل واریانس یک­‌طرفه و در سطح معنی‌­داری 05/0 P<در نظر گرفته شد.

یافته­‌ها

نتایج نشان داد که دویدن با شدت بالا باعث افزایش معنی­‌داری در میزان تروپونین I، LDH و CK قلبی و پلاسمایی شد (0/05P<). همچنین میزان تروپونین I، LDH و CK قلبی و پلاسمایی در گروه سرخ ولیک نسبت به گروه تمرین کاهش معنی­‌داری داشت (0/05P<).

نتیجه­‌گیری

داده‌­ها نشان می‌­دهد عصاره سرخ ولیک احتمالا باعث مهار شاخص‌­های آسیب قلبی رت­‌ها در نتیجه تمرینات استقامتی شدید شود.
کلیدواژه‌ها
آسیب قلب
تمرین
رت ها
سرخ ولیک

عنوان مقاله English

Protective effect Aqueous Extraction of Red Crataegus pentaegyna after High Intensity Running Training on Cardiac Injury Biomarkers in Male rats

نویسندگان English

Ahmad Abdi 1
seyedeh maryam mostafavian 1
maryam davaran 1
tahereh haeri 2
1 Islamic Azad University
2 Ph.D Candidate in Department of Exercise Physiology, University of Mazandaran, Babolsar, Iran
چکیده English

Introduction

The aim of the present study was to examine the protective effect aqueous extract of red Crataegus pentaegyna after high intensity running training on cardiac injury biomarkers in male rats.

Materials and Methods

Twenty Wistar male rats, 140-173 g weight, were randomly classified into saline-control (SC), saline-training (ST), Crataegus-Pentaegyna -control (CPC), and Crataegus-Pentaegyna -training (CPT) groups. Training groups have performed a high-intensity running program (34 m/min, 60 min/day, 5 days/week) on a motor-driven treadmill for eight weeks. The CPC and CPT groups were orally fed with Crataegus-Pentaegyna extraction (500mg/kg) and the same amount of saline was given to the SC and ST groups. The rats were sacrificed seventy- two hours after the last training session. Then, the heart tissue was excised and immediately frozen in liquid nitrogen and stored at -80 °C. The plasma was also collected for measuring the biomarkers. Statistical analysis was performed using a one way analysis of variance, and significance was accepted at p< 0.05.

Results

The results showed the high intensity running significantly increased the levels of cTnI, LDH and CK in heart and plasma of rats. The levels of cTnI, LDH and CK in a CPC in heart and plasma were significantly decreased versus the ST group.

Conclusion

Our findings showed that a Red Crataeguse-Pentaegyna may prevent the cardiac injury biomarkers in rats as a result of intensity endurance training.

کلیدواژه‌ها English

Cardiac Injury
Training
Rats
Red Crataegus
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