تأثیر تمرین مقاومتی بر پروتئین–D سورفکتانت و شاخص مقاومت به انسولین موش‌های صحرایی نر سالم و دیابتی

چکیده
مقدمه

پروتئین– D سورفکتانت (SPD) یک فاکتور جدید مرتبط با عدم تحمل گلوکز، مقاومت به انسولین و دیابت نوع 2 پیشنهاد شده است. ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ اثر 10هفته تمرین مقاومتی بر سطوح سرمی SPD موش‎های صحرایی دیابتی بود.

مواد و روش‌­ها

در این مطالعه تجربی 48 ﺳﺮ موش صحرایی از ﻧﮋاد ویﺴﺘﺎر ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزن 56±200 ﮔﺮم ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ در چهار ﮔﺮوه ﮐﻨﺘﺮل سالم، کنترل دیابتی، سالم تمرین مقاومتی و دیابتی ﺗﻤﺮیﻦ مقاومتی ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺟﻬﺖ اﻟﻘﺎی دیﺎﺑﺖ، از ﻣﺤﻠﻮل نیکوتین آمید به همراه ﻣﺤﻠﻮل استرپتوزوتوسین اﺳﺘﻔﺎده ﮔﺮدیﺪ. گروه‏‌های تمرینی به مدت 10 هفته، برنامه تمرین مقاومتی را اجرا کردند. داده‎ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﺗﺤﻠﯿﻞ واریﺎﻧﺲ یﮏ ﻃﺮﻓﻪ و آزمون تعقیبی Tukeyدر سطح معنی‌­داری 0/05≤ P بررسی شدند.

یافته­‌ها

تمرین مقاومتی موجب کاهش معنی‎دار قند خون ناشتا گروه تمرین مقاومتی دیابتی نسبت به گروه کنترل دیابتی شد (0/043=P). سطوح SPD در گروه کنترل دیابتی نسبت به کنترل سالم کاهش معنی­داری (0/042=P) و شاخص مقاومت به انسولین (HOMA-IR) افزایش معنی­داری (0/024=‎P) یافت. 10 هفته تمرین مقاومتی موجب افزایش معنی­‌دار درSPD در گروه تمرین مقاومتی دیابتی نسبت به گروه کنترل دیابتی شد (0/043=‎P). همچنین تمرین موجب کاهش معنی‌­دار شاخص مقاومت به انسولین (0/034=‎P) و انسولین در گروه تمرین مقاومتی دیابتی نسبت به گروه کنترل دیابتی شد (0/048=‎P)

نتیجه­‌گیری

نتایج حاصل از این مطالعه به نظر می­‌رسد تأیید کننده نقش پروتئین–D سورفکتانت مرتبط با تمرین مقاومتی در بهبود شاخص مقاومت به انسولین در موش‎های صحرایی دیابتی نوع 2 می‏‌باشد.
کلیدواژه‌ها
موش صحرایی
استرپتوزوتوسین- نیکوتین‌آمید
SPD
انسولین
تمرین مقاومتی

عنوان مقاله English

The effect of resistance training on protein-D surfactant and insulin resistance index in healthy and type 2 diabetic rats

چکیده English

Introduction

Protein-D Surfactant (SPD) is a new factor associated with glucose intolerance, insulin resistance, and type 2 diabetes. The aim of the present study was to investigate the effects of resistance training on surfactant protein-D in streptozotocin-nicotinamide-induced diabetic rats.

Materials and Methods

In this experimental study، 48 adult male Wistar rats in the weight range of 200±48gr, randomly assigned into four groups: healthy control, diabetic control, diabetic resistance training, and healthy resistance training. For induction of diabetes, intraperitoneal injection of nicotinamide solution and STZ solution was used. The training groups performed a resistance training program for 10 weeks. Data were analyzed using one-way ANOVA and Tuky Post-hoc test at 0.05%.

Results

Resistance training caused a significant decrease in blood glucose in the resistance diabetic training group compared to the diabetic control group (P=0.024). Serum Protein-D Surfactant (SPD) serum levels in the diabetic control group significantly decreased (P=0.042) and the insulin resistance index (P=0.024) was significantly lower than healthy controls. 8 weeks of endurance training significantly increased in SPD in endurance diabetic training group compared to diabetic control group (P=0.043). Also, exercise significantly decreased insulin resistance index (P=0.034) and insulin in endurance diabetic training group compared to diabetic control group (P=0.046).

Conclusion

The results of this study seem to confirm the role of Protein-D Surfactant associated with resistance training in improving insulin resistance index in type 2 diabetic rats.

کلیدواژه‌ها English

rat
Streptozotocin–nicotinamide
SPD
Insulin
resistance training
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