اثر محافظتی تمرین هوازی همراه با مکمل رزوراترول بر بیان برخی شاخص‌های آتروفی کاردیومیوسیت‌های رت‌های دیابتی

نویسندگان
بنت الهدی اسماعیلی 1
احمد عبدی 1
پروین فرزانگی 2
آسیه عباسی دلویی 1
1 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
چکیده
مقدمه

هدف از پژوهش حاضر بررسی اثر تمرین هوازی همراه با مصرف رزوراترول بر بیان FOXO3a و MuRF1 بافت قلب رت­‌های نر دیابتی می‌باشد.

مواد و روش‌­ها

40 سر رت نر ویستار، به طور تصادفی به گروه‌های کنترل- سالم (CN)، دیابت (DM)، دیابت-رزوراترول (RDM)، دیابت-تمرین هوازی (TDM)، دیابت-تمرین هوازی-رزوراترول (TRDM) تقسیم شدند. گروه‌های تمرینی به مدت 8 هفته برنامه تمرینی دویدن را روی تردمیل انجام دادند. به گروه‌های RDMو TRDM (20 میلی‌گرم/کیلوگرم) رزوراترول تزریق شد. 48 ساعت پس از آخرین جلسه تمرینی، رت‌ها کشتار شدند، بافت قلب برداشته و بلافاصله در محلول نیتروژن فریز و در دمای 80- برای اندازه‌گیری شاخص‌ها نگهداری شد. برای تجزیه و تحلیل آماری از تحلیل واریانس در سطح معنی‌داری 0/05≤ p استفاده شد.

یافته­‌ها

نتایج پژوهش حاضر نشان دهنده کاهش بیان FOXO3a (0001/0p=) و MuRF1 (0001/0p=) کاردیومیوسیت‌ها در گروه‌های TDM نسبت به گروه DM بود. بیان FOXO3a (045/0p=) و MuRF1 (037/0p=) در گروه RDM نسبت به گروه DM کاهش معنی‌داری داشت. همچنین بیان FOXO3a (026/0p=) و MuRF1 (007/0p=) کاردیومیوسیت‌ها در گروه‌های TRDM نسبت به گروه DM نیز کاهش معنی‌داری داشت.

نتیجه­‌گیری

یافته‌های ما نشان داد تمرین هوازی با و بدون رزوراترول احتمالا باعث مهار شاخص‌های آتروفی کاردیومیوسیت­‌های رت­‌های دیابتی می‌شود.
کلیدواژه‌ها
تمرین
گیاه دارویی
FOXO3a و MuRF1
موش های صحرایی

عنوان مقاله English

Protective Effect of Aerobic Training along with Resveratrol on the Expression of some Atrophic Biomarkers of Cardiomyocytes in Diabetic rats

نویسندگان English

Bentollhoda Esmailee 1
Ahmad Abdi 1
parvin farzanegi 2
Asieh Abbassi Daloii 1
1 Islamic Azad University
2 Islamic Azad University
چکیده English

Introduction

The aim of the present study was to examine the effect of aerobic training along with resveratrol on cardiac expression of FOXO3a and MuRF1 in diabetic male rats.

Materials and Methods

Forty Wistar male rats were randomly classified into Control-Normal (CN), Diabetes Mellitus (DM), Resveratrol-Diabetes Mellitus (RDM), Training-Diabetes Mellitus (TDM) and Training-Resveratrol-Diabetes Mellitus (TRDM) groups. Training groups have performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the RDM and TRDM groups. Forty-eight hours after the last training session, rats were sacrificed; hearts were excised and immediately frozen in liquid nitrogen and stored at -80 °C for measuring the indexes. Statistical analysis was performed using a one-way analysis of variance, and significance was accepted at p ≤ 0.05.

Results

The results of this study showed a reduction in the expression of FOXO3a (p=0.0001) and MuRF1 (p=0.0001) cardiomyocytes in the TDM group compared to the DM group. The expression of FOXO3a (p=0.045) and MuRF1 (p=0.037) in the RDM group was significantly decreased in comparison to the DM group. Also, the expression of FOXO3a (p=0.026) and MuRF1 (p=0.007) cardiomyocytes in the TRDM groups also had a significant decrease compared to the DM group.

Conclusion

Our findings show that an aerobic training with and without resveratrol maybe inhibits atrophic biomarkers of cardiomyocytes in diabetic male rats.

کلیدواژه‌ها English

Exercise
herbs
FOXO3a
MuRF1
Rats
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