اثر گالیک اسید بر زنده‌مانی جنین وآسیب سلولی هیپوکامپ در مدل حیوانی نارسایی رحمی – جفتی

نویسندگان
محمد یوسفی قلاتی
محمد امین عدالت منش
گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
چکیده
مقدمه

نارسایی رحمی- جفتی (Utreoplacental Inssuficiency; UPI) سبب آسیب مغزی و اختلالات تکاملی در جنین­ دچار محدودیت رشد درون رحمی می­گردد. مطالعه حاضر به بررسی اثر گالیک اسید بر زنده­مانی و وزن جنین، رحم و جفت و دانسیته سلول­های هرمی هیپوکامپ جنین­ در مدل UPI می­پردازد.

مواد و روش­ها

تعداد 25 سر موش صحرایی باردار نژاد ویستار به صورت تصادفی در 5 گروه تقسیم شدند: گروه کنترل، UPI+NS (نارسایی رحمی-جفتی+ نرمال سالین)، UPI+GA100 (نارسایی رحمی-جفتی+ گالیک اسید 100)، UPI+GA200 (نارسایی رحمی-جفتی+ گالیک اسید 200) و UPI+GA400 (نارسایی رحمی-جفتی+ گالیک اسید 400). القاء UPI با انسداد شریان های قدامی رحمی در روز 18 بارداری القاء شد. تجویز گالیک اسید یا نرمال سالین از روز 15 تا 21 بارداری به روش گاواژ انجام شد. سپس، در روز 21 بارداری جنین­ها سزارین شده و پس از توزین، به روش هایپوترمی بیهوش، سر آنها قطع و مغز جهت مطالعات هیستوپاتولوژیک مورد ارزیابی قرار گرفتند.

یافته­ها

افزایش معنی­دار وزن رحم و کاهش معنی­دار درصد زنده­مانی جنین و دانسیته سلولی در نواحی CA1، CA2 و CA3 هیپوکامپ جنین در گروه UPI+NS نسبت به گروه کنترل دیده شد (05/0˂p). از طرفی، درصد زنده­مانی، وزن جنین و دانسیته سلولی در نواحی CA1 و CA3 در گروه­های تیمار شده با گالیک اسید نسبت به گروه UPI+NS افزایش معنی­داری را نشان داد (05/0˂p).

نتیجه­گیری

گالیک اسید، سبب کاهش مرگ و میر جنین و آسیب سلولی هیپوکامپ جنین در مدل UPI گردید. از این رو، می­تواند از عوارض تکاملی عصبی ناشی از محدودیت رشد درون رحمی پیشگیری نماید.
کلیدواژه‌ها
گالیک اسید
محدودیت رشد جنینی
آسیب مغزی
هیپوکامپ
زنده‌مانی جنین

عنوان مقاله English

The effect of Gallic acid on fetus viability and hippocampal cell damages in rat model of utreo-placental insufficiency

نویسندگان English

Mohammad Yousefi-Ghalati
Mohammad Amin Edalatmanesh
Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
چکیده English

Introduction

Uteroplacental insufficiency (UPI) causes brain damage and neurodevelopmental deficits in intrauterine growth-restricted fetus. This study examined the effect of Gallic acid (GA) on fetus viability and weight of fetus, uterus, and placenta and hippocampal piramidal cell densityof fetus in UPI model.

Materials and Methods

25 pregnant Wistar rats were randomly divided into 5 groups: control, UPI+NS (Uteroplacental insufficiency + Normal saline), UPI+GA100 (Uteroplacental insufficiency + Gallic acid 100), UPI+GA200 (Uteroplacental insufficiency + Gallic acid 200) and UPI+GA400 (Uteroplacental insufficiency + Gallic acid 400). UPI induction is carried out by anterior uterine artery occlusion on gestation day (GD) 18. GA or normal saline was administrated by gavage method from GD15 to GD21. Then, on GD21 the fetus were cesarean section and after weighing, anesthetized by hypothermia, their heads were cut and the brains evaluated for histopathological studies.

Results

A significant increase in uterine weight and a significant decrease in fetal viability and cell density were observed in the hippocampal CA1, CA2 and CA3 subdivision in UPI+NS group compared to the control group (p˂0.05). On the other hand, viability rate, fetus weight and cell density in CA1 and CA3 subdivisions were significantly higher in the GA-treated groups than the UPI+NS group (p˂0.05).

Conclusion

GA reduced fetal mortality and fetal hippocampal cell damage in UPI model. Therefore, it usable to avoid the neurodevelopmental complications due to intrauterine growth restriction.

کلیدواژه‌ها English

Gallic acid
Fetal Growth Retardation
Brain injuries
Hippocampus
Fetal viability
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