اثر فعال‌سازی سیستم ایمنی بدن مادر بر بیان مارکرهای التهابی استریاتوم مغز فرزندان نر متولد شده در مدل تجربی اسکیزوفرنیا القا شده با لیپوپلی‌ساکارید

نویسندگان
روناک موسویان 1
ریحانه بابایی 2
فائزه علیزاده 2
ابراهیم سواره 2
هانیه کاظمی 1
ناهید داودیان 3
1 مرکز تحقیقات پزشکی مولکولی، پژوهشکده سلامت هرمزگان، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران
2 گروه بیوشیمی، دانشکده پزشکی، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران
3 مرکز تحقیقات غدد و متابولیسم، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران
چکیده
مقدمه

اخیرا مطالعات اپیدمیولوژیک و همچنین مدل‌های تجربی حیوانی، عفونت دوران بارداری را به عنوان یک فاکتور خطر مهم در بروز بیماری اسکیزوفرنیا در فرزندان شناسایی کرده‌اند. علاوه بر این، مطالعات زیادی ارتباط بین اسکیزوفرنیا و التهاب را نشان داده‌اند. در مطالعه حاضر، با استفاده از مدل فعالسازی سیستم ایمنی مادر (MIA) القا شده با لیپوپلی‌ساکارید (LPS)، اثر MIA بر بیان چند مارکر التهابی در استریاتوم فرزندان نر متولد شده مورد بررسی قرار گرفت

مواد و روش­ها

موش‌های باردار در روزهای 15 و 16 بارداری به صورت درون صفاقی سالین و یا LPS با دوز 500 میکروگرم به ازای کیلوگرم وزن بدن را دریافت کردند. شصت روز پس از تولد، فرزندان نر بدنیا آمده یوتانزی شده و بیان ژن ماکرهای التهابی در استریاتوم آنها مورد ارزیابی قرار گرفت.

یافته­ها

بیان ژن‌های NF-κβ، IL-6، IL-1β وTNFα در استریاتوم فرزندان نر بدنیا آمده از مادران عفونی شده نسبت به گروه کنترل افزایش معناداری داشت.

نتیجه­گیری

بیان ژن‌های NF-κβ، IL-6، IL-1β و TNFα در استریاتوم فرزندان نر بدنیا آمده از مادران عفونی شده نسبت به گروه کنترل افزایش معناداری داشت.
کلیدواژه‌ها
اسکیزوفرنیا
مارکرهای التهابی
استریاتوم
فعال‌سازی سیستم ایمنی مادر
لیپوپلی‌ساکارید

عنوان مقاله English

The effect of maternal immune activation on the expression of inflammatory factors in the striatum of male offspring in lipopolysaccharide-induced experimental model of schizophrenia

نویسندگان English

Ronak Mousavian 1
Reyhaneh Babaei Khorzoughi Babaei Khorzoughi 2
Faezeh Alizadeh 2
Ebrahim Savare 2
Haniyeh Kazemi 1
Nahid Davoodian 3
1 Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2 Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3 Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
چکیده English

Introduction

Recently, epidemiological studies, as well as experimental animal models, have identified the gestational infection as a particular risk factor for the development of schizophrenia in the offspring. Besides, several studies have demonstrated the association between schizophrenia and inflammation. Using a rat model of maternal immune activation (MIA) induced by lipopolysaccharide (LPS), the present study evaluated the effect of MIA on the expression of several pro-inflammatory markers in the striatum of male offspring.

Materials and Methods

Pregnant rats were intraperitoneally administered with either saline or LPS (500µg/kg) on gestation days 15 and 16. At postnatal day 60, male offspring were humanely killed, and the gene expression of inflammatory mediators was evaluated in the striatum.

Results

Male rats born to LPS-challenged mothers showed significantly increased expression of NF-κβ، IL-6، IL-1β, and TNFα in the striatum compared to the control group.

Conclusion

Collectively, these results support the idea that prenatal immune challenge induces increased inflammatory markers in the striatum of the resulting offspring.

کلیدواژه‌ها English

Schizophrenia
pro-inflammatory markers
striatum
maternal immune activation
lipopolysaccharide
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