مقایسه اثر یک دوره تمرین هوازی و ازن‌درمانی بر بیان ژن Beclin-1، AMPKو mTOR بافت غضروف در موش‌های صحرایی مبتلا به استئوآرتریت زانو

نویسندگان
آزاده نصرت پور 1
پروین فرزانگی 2
رضا رضایی 1
1 گروه تربیت بدنی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران
2 گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
چکیده
مقدمه

هدف از انجام مطالعه حاضر، تعیین اثر هشت هفته تمرین هوازی و ازن درمانی بر بیان ژن Beclin-1، mTOR و AMPK بافت غضروف آسیب دیده در موش­های صحرایی مبتلا به استئوآرتریت (OA) زانو بود.

مواد و روش­ها

در این مطالعه تجربی، 40 سر موش صحرایی نر ویستار 10±220 گرمی به طور تصادفی به پنج گروه سالم (CN)، استئوآرتریت (OA)، OA+ازن درمانی، OA+تمرین هوازی و OA+ازن درمانی+تمرین هوازی تقسیم شدند. استئوآرتریت به روش جراحی انجام شد. تمرین ورزشی شامل دویدن روی نوارگردان به مدت هشت هفته، پنج جلسه در هفته، هر جلسه 35-45 دقیقه و با سرعت 18 متر در دقیقه بود. موش­های گروه ازن درمانی، ازن (O3) با غلظت 20 پیکوگرم بر لیتر، یک بار در هفته و در مدت سه هفته دریافت نمودند. 48 ساعت پس از آخرین جلسه تمرین و در وضعیت 12 ساعت ناشتایی نمونه­های غضروف زانو جدا شد و سطوح mRNA فاکتورهای Beclin-1، AMP و mTOR به روش RT- PCRاندازه­گیری شد.

یافته­ها

در بافت غضروفی، سطوح Beclin-1 در گروه OA کاهش، ولیکن AMPKو mTOR افزایش یافت. ازن درمانی، تمرین هوازی و ترکیب ازن درمانی+تمرین هوازی موجب معکوس شدن روند تغییرات شد (0.009=P). اما بین این گروه­ها تفاوت معناداری وجود نداشت (0.05≥P).

نتیجه­گیری

بر اساس نتایج، ازن درمانی، تمرین ورزشی و ترکیبی سطوح mRNA فاکتورهای Beclin-1 غضروف مفصلی را افزایش می­دهند. اما، تمرین ورزشی با شدت متوسط با تعدیل سطوح mTOR ممکن است نقش بیشتری در کنترل اتوفاژی کندروسیت غضروف مفصلی در موش­های صحرایی مدل OA داشته باشد.
کلیدواژه‌ها
تمرین هوازی
ازن درمانی
استئوآرتریت
Beclin-1
AMPKو mTOR

عنوان مقاله English

The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis

نویسنده English

parvin rezaee 2
1 Department of Physical Education, Aliabad Katoul branch, Islamic Azad University, Aliabad Katoul, Iran
2 Department of sport physiology, Sari branch, Islamic Azad University, Sari, Iran
چکیده English

Introduction

The aim of the research was to investigate the effect of 8 weeks’ exercise training and ozone therapy on articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression in rat’s model of osteoarthritis (OA).

Materials and Methods

In this experimental study, 40 adult male rats 210±10 gr were randomly divided into 5 groups: 1) control (CN), 2) OA, 3) OA + ozone, 4) OA + exercise training and 5) OA + ozone + exercise training groups. The knee OA was induced by the surgical method in rats. The exercise training included running on a treadmill for 8 weeks, five times a week, and 35-45 minutes in an exercise session and with 18m per minute speed.

Rats in the ozone group received ozone (O3) at a concentration of 20μg/ml, once weekly for 3 weeks. 48 hours after the programs and fasting, cartilage tissues were isolated, and articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression were considered using Real-Time PCR (RT-PCR).

Results

In cartilage tissue, Beclin-1levels had a significant decrease in the OA group, but AMPK and mTOR levels increase. Ozone, exercise training, and ozone + exercise training Caused to reverse the process (P=0.009). But, there was no significant difference between these groups (P≥0.05).

Conclusion

According to the results of this study, ozone therapy and moderate-intensity exercise training Increase Beclin-1levels in the articular cartilage tissue. However, moderate-intensity exercise training with adjustable levels of mTOR may Play a greater role in the control of articular chondrocyte autophagy in rat’s model of OA.

کلیدواژه‌ها English

Aerobic Exercise
Ozone Therapy
Osteoarthritis
Beclin-1
AMPK
mTOR
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