The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis

Author
parvin rezaee
Department of sport physiology, Sari branch, Islamic Azad University, Sari, Iran
Abstract
Introduction

The aim of the research was to investigate the effect of 8 weeks’ exercise training and ozone therapy on articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression in rat’s model of osteoarthritis (OA).

Materials and Methods

In this experimental study, 40 adult male rats 210±10 gr were randomly divided into 5 groups: 1) control (CN), 2) OA, 3) OA + ozone, 4) OA + exercise training and 5) OA + ozone + exercise training groups. The knee OA was induced by the surgical method in rats. The exercise training included running on a treadmill for 8 weeks, five times a week, and 35-45 minutes in an exercise session and with 18m per minute speed.

Rats in the ozone group received ozone (O3) at a concentration of 20μg/ml, once weekly for 3 weeks. 48 hours after the programs and fasting, cartilage tissues were isolated, and articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression were considered using Real-Time PCR (RT-PCR).

Results

In cartilage tissue, Beclin-1levels had a significant decrease in the OA group, but AMPK and mTOR levels increase. Ozone, exercise training, and ozone + exercise training Caused to reverse the process (P=0.009). But, there was no significant difference between these groups (P≥0.05).

Conclusion

According to the results of this study, ozone therapy and moderate-intensity exercise training Increase Beclin-1levels in the articular cartilage tissue. However, moderate-intensity exercise training with adjustable levels of mTOR may Play a greater role in the control of articular chondrocyte autophagy in rat’s model of OA.
Keywords
Aerobic Exercise
Ozone Therapy
Osteoarthritis
Beclin-1
AMPK
mTOR
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Journal of Netshabur University of Medical Sciences
Volume 8, Issue 4 - Serial Number 29
Autumn 2020
Pages 124-135