Evaluation the effects of Teucrium polium hydroalcoholic extract on the fetus and chorioallantoic fluid using a chick embryo model

Authors
Amin Derakhshanfar 1
Hadi Tavakkoli 2
Javad Moayedi 3
Ali Poostforoosh Fard 4
1 Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran; Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Clinical Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
3 Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
4 D.V.M., Vice Chancellery for Research Affairs, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Introduction

Side effects of drugs have always been controversial issues in medical science. To date, sufficient researches were not performed on the side and pathological effects of Teucrium polium as an herb in the fetus. Therefore, the present study aimed to investigate the pathological effects of Teucrium polium hydroalcoholic extract on chorioallantoic fluid composition, weight, growth, external and internal tissues of the fetus using a chick embryo model.

Materials and Methods

A total of 48 Ross 308 embryonated eggs were randomly divided into 3 equal groups. In the first group (control), 0.5 CC of phosphate buffer saline and the second and third groups, Teucrium polium hydroalcoholic extract with doses of 25 and 50 mg/kg egg-weight were inoculated into the eggs. Embryos were allowed to develop until day 18 and then examined for macroscopic and microscopic lesions.

Results

Injection of e hydroalcoholic extract caused a disturbance in the weight, growth, and biochemical factors of the chorioallantoic fluid during the growth period. The most pathological effects were observed in the brain, lung, heart, liver, and kidney of the embryos which received the Teucrium polium hydroalcoholic extract at a dose of 50 mg/kg.

Conclusion

Teucrium polium hydroalcoholic extract can produce various pathological effects in the external organ, internal organ, and the chorioallantoic fluid. Considering the consumption of this herb in different regions of Iran, it is recommended that its usage be taken with caution, especially during pregnancy.
Keywords
Histopathology
Herbal
Teucrium polium
Fetus
Chick
1. De Marino S, Festa C, Zollo F, Incollingo F, Raimo G, Evangelista G, et al. Antioxidant activity of phenolic and phenylethanoid glycosides from Teucrium polium L. Food Chem. 2012;133(1):21-8.
2. Goulas V, Gomez-Caravaca AM, Exarchou V, Gerothanassis IP, Segura-Carretero A, Gutiérrez AF. Exploring the antioxidant potential of Teucrium polium extracts by HPLC–SPE–NMR and on-line radical-scavenging activity detection. LWT-Food Sci Technol. 2012;46(1):104-9.
3. Khodadadi S, Zabihi NA, Niazmand S, Abbasnezhad A, Mahmoudabady M, Rezaee SA. Teucrium polium improves endothelial dysfunction by regulating eNOS and VCAM-1 genes expression and vasoreactivity in diabetic rat aorta. Biomed Pharmacother. 2018;103(1):1526-30.
4. Salimnejad R, Sazegar G, Borujeni MJS, Mousavi SM, Salehi F, Ghorbani F. Protective effect of hydroalcoholic extract of Teucrium polium on diabetes-induced testicular damage and serum testosterone concentration. Int J Reprod Biomed. 2017;15(4):195-202.
5. Tabatabaie PS, Yazdanparast R. Teucrium polium extract reverses symptoms of streptozotocin-induced diabetes in rats via rebalancing the Pdx1 and FoxO1 expressions. Biomed Pharmacother. 2017;93(1):1033-9.
6. Shahraki MR, Arab MR, Mirimokaddam E, Palan MJ. The effect of Teucrium polium (Calpoureh) on liver function, serum lipids and glucose in diabetic male rats. Iran Biomed J. 2007;11(1):65-8.
7. Savvidou S, Goulis J, Giavazis I, Patsiaoura K, Hytiroglou P, Arvanitakis C. Herb-induced hepatitis by Teucrium polium L.: report of two cases and review of the literature. Eur J Gastroenterol Hepatol. 2007;19(6):507-11.
8. Dourakis SP, Papanikolaou IS, Tzemanakis EN, Hadziyannis SJ. Acute hepatitis associated with herb (Teucrium capitatum L.) administration. Eur J Gastroenterol Hepatol. 2002;14(6):693-5.
9. Bahramikia S, Yazdanparast R. Phytochemistry and medicinal properties of Teucrium polium L.(Lamiaceae). Phytother Res. 2012;26(11):1581-93.
10. Rafieian-Kopaei M, Nasri H, Baradaran A. Teucrium polium: Liver and kidney effects. J Res Med Sci. 2014;19(5):478-9.
11. Amraei M, Ghorbani A, Seifinejad Y, Mousavi SF, Mohamadpour M, Shirzadpour E. The effect of hydroalcoholic extract of Teucrium polium L. on the inflammatory markers and lipid profile in hypercholesterolemic rats. J Inflamm Res. 2018;11(1):265-72.
12. Sharifian Z, Vodgani M, Kamalinejad M. Immunological effects of Teucrium polium on neutrophils. Iran J Pharm Res. 2010;3(2):61-.
13. Khazaei M, Nematollahi-Mahani SN, Mokhtari T, Sheikhbahaei F. Review on Teucrium polium biological activities and medical characteristics against different pathologic situations. J Contemp Med Sci. 2018;4(1):1-6.
14. Sadraeisadraei H, Hajhashemi V, Ghannadi A, Mohseni M. Antispasmodic effect of aerial part of Teucrium polium L. essential oil on rat isolated ileum in vitro. Med J Islam Repub Iran. 2001;14(4):355-8.
15. Singroha R, Srivastava S, Chhikara P. Effect of Gentamicin on kidney in developing chicks. Eur J Anat. 2012;16(2):119-26.
16. DeBord LC, Pathak RR, Villaneuva M, Liu H-C, Harrington DA, Yu W, et al. The chick chorioallantoic membrane (CAM) as a versatile patient-derived xenograft (PDX) platform for precision medicine and preclinical research. Am J Cancer Res. 2018;8(8):1642-60.
17. Kain KH, Miller JW, Jones‐Paris CR, Thomason RT, Lewis JD, Bader DM, et al. The chick embryo as an expanding experimental model for cancer and cardiovascular research. Dev Dyn. 2014;243(2):216-28.
18. Tavakkoli H, Derakhshanfar A, Moayedi J, Fard AP, Behrouz S, Piltan MA, et al. Evaluation of the toxicopathological lesions of Calotropis procera using a chick embryonic model. Comp Clin Path. 2019;28(1):195-202.
19. Tavakkoli H, Derakhshanfar A, Noori Gooshki S. Toxicopathological lesions of fosfomycin in embryonic model. Eur J Exp Biol. 2014;4(2):63-71.
20. Tavakkoli H, Derakhshanfar A, Noori Gooshki S. The effect of florfenicol egg-injection on embryonated chicken egg. Int J Adv Biol Biomed Res. 2014;2(2):496-503.
21. Tavakkoli H, Derakhshanfar A, Noori Gooshki S. A short preliminary experimental study on teratogenic effect of methenamine in embryonic model. Int J Adv Biol Biomed Res. 2013;1(12):1523-8.
22. Venditti A, Frezza C, Trancanella E, Zadeh SMM, Foddai S, Sciubba F, et al. A new natural neo-clerodane from Teucrium polium L. collected in Northern Iran. Ind Crops Prod. 2017;97(1):632-8.
23. Safaeian L, Ghanadian M, Shafiee-Moghadam Z. Antihyperlipidemic effect of different fractions obtained from Teucrium polium hydroalcoholic extract in rats. Int J Prev Med. 2018;9(1):30-5.
24. Amraei M, Ghorbani A, Seifinejad Y, Mousavi SF, Mohamadpour M, Shirzadpour E. The effect of hydroalcoholic extract of Teucrium polium L. on the inflammatory markers and lipid profile in hypercholesterolemic rats. J Inflamm Res. 2018;11(2018):265—72.
25. Baradaran A, Madihi Y, Merrikhi A, Rafieian-Kopaei M, Nematbakhsh M, Asgari A, et al. Nephrotoxicity of hydroalcoholic extract ofTeucrium polium in Wistar rats. Pak J Med Sci. 2013;29(1):329-33.
26. Nasri H, Rafieian-Kopaei M. Nephrotoxicity of hydroalcoholic extract of teucrium polium in wistar rats. J Isfahan Med School. 2014;32(290):938-46.
27. Esen SA, Kahvecioğlu S, Gül CB, Aktaş N, Esen İ. Toxic effects of herbal medicines: Teucrium polium and acute kidney injury. Eur Respir J. 2019;5(6):1028-30.
28. Vargas A, Zeisser-Labouèbe M, Lange N, Gurny R, Delie F. The chick embryo and its chorioallantoic membrane (CAM) for the in vivo evaluation of drug delivery systems. Adv Drug Deliv Rev. 2007;59(11):1162-76.
29. Smith SM, Flentke GR. The Avian Embryo as a Model for Fetal Alcohol Spectrum Disorders. Biochem Cell Biol. 2018;96(2):98-106.
Journal of Netshabur University of Medical Sciences
Volume 8, Issue 4 - Serial Number 29
Autumn 2020
Pages 136-147