سنجش عملکرد حافظه موش های صحرائی نوزاد متولد از مادران تحت درمان با بوپرنورفین در دوران بارداری و شیردهی

نویسندگان
سعید سمرقندیان 1
امید مهرپور 2
محسن فوادالدینی 3
بابک روشن روان 4
طاهره فرخنده 5
1 مرکز تحقیقات بیماریهای غیر واگیر، دانشگاه علوم پزشکی نیشابور، نیشابور، ایران
2 مرکز دارو و سم شناسی راکی، دنور، آمریکا؛ کمیته تحقیقات دانشجویی ، دانشگاه علوم پزشکی بیرجند ، بیرجند ، ایران
3 مرکز تحقیقات قلب و عروق، دانشگاه علوم پزشکی بیرجند، بیرجند ایران
4 کمیته تحقیقات دانشجویی ، دانشگاه علوم پزشکی بیرجند ، بیرجند ، ایران
5 مرکز تحقیقات قلب و عروق، دانشگاه علوم پزشکی بیرجند، بیرجند ایران؛ دانشکده داروسازی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران
چکیده
مقدمه

بوپرنورفین از جمله داروهای اپیوئیدی است که به‌عنوان درمان نگهدارنده اعتیاد در دوران بارداری و شیردهی تجویز می‌گردد، با این وجود اثرات سو آن بر جنین و نوزاد شیرخوار به‌خوبی بررسی نگردیده است؛ بنابراین در مطالعه حاضر به بررسی اثر تجویز بوپرنورفین در دوران بارداری و شیردهی بر عملکرد حافظه موش­های نوزاد می‌پردازیم.

مواد و روش­ها

سی سر از موش‌های باردار و موش‌های شیرده در دو گروه کنترل و چهار گروه درمانی به طور تصادفی قرار داده شدند (n=5 برای هر گروه مادر و n=6 برای هر گروه نوزاد که گروه هدف مورد بررسی است). گروه کنترل شامل موش‌های صحرایی ماده باردار و شیرده که به طور زیر جلدی یک دوز حامل دریافت می‌کردند و گروه‌های درمانی شامل ماده‌های باردار و شیردهای بودند که به طور زیر جلدی بوپرنورفین 5/0 و 1 میلی‎‌گرم در کیلوگرم یک بار در روز دریافت می‌کردند. نوزادان 28 روز بعد از زایمان از شیر گرفته شدند و 6 نوزاد از هر گروه به طور تصادفی جهت بررسی روند حافظه انتخاب گردید. روند حافظه با استفاده از آزمون Y-maze بررسی گردید.

یافته­ها

درصد تناوب حاصل تفاوت معنی­داری بین گروه­های مواجه شده با بوپرنورفین در دوره جنینی و شیرخواری با گروه کنترل نشان نداد.

نتیجه­گیری

مواجهه با بوپرنورفین در دوره جنینی و شیرخواری احتمالاً تاثیری بر عملکرد حافظه فضایی به همراه ندارد با این وجود، مطالعات بیشتری جهت نشان دادن تاثیر بوپرنورفین بر فعالیت‌های شناختی لازم می‌باشد

کلیدواژه‌ها
بوپرنورفین
حافظه
دوره جنینی
دوره شیرخورای
تست عملکرد حافظه

عنوان مقاله English

Assessment of memory function of offspring rats born to mothers under treating with buprenorphine during pregnancy and lactation

نویسندگان English

Saeed Samarghandian 1
Omid Mehrpour 2
Moshen Foaddini 3
Babak Roshanravan 4
Tahereh Farkhondeh 5
1 Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran
2 Rocky Mountain Poison and Drug Safety, Denver Health, Denver, CO 80204, USA; Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
3 Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
4 Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
5 Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran; Faculty Pharmacy, Birjand University of Medical Sciences, Birjand, Iran
چکیده English

Introduction: Buprenorphine is an opioid drug that is prescribed as an opioid maintenance therapy for addiction during pregnancy and lactation, but its adverse effects on the fetus and infant have not been well studied. Therefore, in the present study, we investigated the effect of buprenorphine administration during pregnancy and lactation on the memory function of offspring animals.

Method: 30 female rats in gestation and lactation stages are allocated in the controls, gestation and lactation treat groups. After parturition, dams and their pups were kept in one cage during lactation without BUP administration. After parturition, other groups subcutaneously receiving one dose of vehicle as a control group, and lactation treat group (0.5 and 1 BUP) subcutaneously receiving BUP 0.5 and 1 mg/kg once a day and pups are weaned on postnatal (PN) day 28. Six offspring from each group were randomly selected to evaluate the memory function. The spatial memory function was checked using the Y-maze test.

Results: In this study, there was no significant difference between the groups exposed to buprenorphine in the embryonic and breastfeeding period and the control group in the alternative percentage.

Conclusion: The present study showed that exposure to buprenorphine in the fetal and infant stage has no effect on the spatial memory function, but this study does not confirm the safety of this drug and further studies are needed to show the effect of buprenorphine on cognitive function.

کلیدواژه‌ها English

Buprenorphine
Memory
fetal period
lactation
memory function test
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