تأثیر یک دوره تمرین ترکیبی بر بیان ژن ABCB4 گلبول‌های سفید و نسبت HDL به LDL در مردان میانسال بعد از انجام عمل بای‌پس عروق قلبی

نویسندگان
ربابه محمودی 1
امیر رشیدلمیر 2
رامبد خواجه ای 1
آمنه برجسته یزدی 1
1 گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
2 گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران
چکیده
مقدمه

بیماری‌های قلبی و عروقی در سراسر جهان و از جمله ایران شیوع زیادی دارند و در این بین تصلب شرایین عامل عمده مرگ و میر در تمام دنیا محسوب می‌شود. هدف پژوهش حاضر بررسی تاثیر یک دوره تمرین ترکیبی بر بیان ژن ABCB4 و نسبت HDL به LDL در مردان میانسال بعد از انجام عمل بای‌پس عروق قلبی می‌باشد.

مواد و روش­ها

نمونه آماری این تحقیق را 30 مرد میانسال تشکیل دادند که قبلاً عمل بای‌پس عروق کرونری انجام داده بودند( بیماران CABG). این تحقیق از نوع نیمه تجربی است که به روش نمونه گیری انتخابی به‌طور تصادفی در دو گروه کنترل(15نفر), تمرین ترکیبی استقامتی و مقاومتی، 15 نفر) قرار گرفتند. میانگین وزن آزمودنی‌ها 5.87± 75.45 (کیلوگرم) و شاخص توده بدن 56/1± 11/25 (وزن×قد²) بود. قبل و بعد جلسه تمرینی (24 جلسه ‌3 روز در هفته) از آزمودنی‌ها خون‌گیری به عمل آمد. پس از جداسازی گلبول‌‎های تک هسته‌ای با استفاده از فایکول و تخلیصmRNA تغییرات بیان ژن به‌ روش Real-Time PCr انجام شد. داده‌ها با آزمون آماری تحلیل واریانس با اندازه‌های تکراری تجزیه و تحلیل شد.

یافته‌­ها

هشت هفته تمرین منجر به افزایش معنی‌داری در بیان ژن ABCB4 و کاهش معنی‌دار سطوح پلاسمایی کلسترول تام گروه تمرین ترکیبی در مقایسه با گروه کنترل شد.

نتیجه‌­گیری

به نظر می‌رسد تمرین ترکیبی به عنوان بخشی از مراحل بازتوانی قلبی باعث افزایش بیان ژن ABCB4 و انتقال معکوس کلسترول و کاهش سطوح پلاسمایی کلسترول تام و بهبود شرایط آندوتلیالی دربیماران مرد میانسال بعد از انجام عمل بای‌پس عروق قلبی می‌شود.
کلیدواژه‌ها
عمل بای‌پس قلبی
تمرین ترکیبی
ژن ABCB4

عنوان مقاله English

The effect of combined training on lipid profile of white blood cells ABCB4 gene expression and HDL to LDL ratio in middle- aged men after coronary artery bypass graft

نویسندگان English

Robabe Mahmoodi 1
Amir Rashidlamir 2
Rambod Khajeie 1
Ameneh Barjaste Yazdi 1
1 Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده English

Introduction

Cardiovascular diseases are very common all over the world, including Iran. Meanwhile, atherosclerosis is the leading cause of death worldwide. The aim of this study was to investigate the effect of combined training on ABCB4 gene expression and HDL to LDL ratio in middle-aged men after coronary artery bypass graft.

Materials and Methods

The statistical population consisted 30 middle-aged men that had previously undergone coronary artery bypass graft. This research is quasi-experimental which by random sampling method, they were randomly divided into two groups of control (n=15) and combined training (endurance and resistance, n=15). Blood samples were taken from the two groups before and after the training session. After isolation mononuclear cells by using follicles and mRNA purification, gene expression variation was performed by Real- Time PCR. Data were analyzed by repeated measures variance analysis with SPSS software version 16.

Results

Eight weeks of training resulted in a significant increase in ABCB4 gene expression and significant reduction in plasma total cholesterol levels in the combined training group compared to the control group.

Eight weeks of training resulted in a significant increase in ABCB4 gene expression and significant reduction in plasma total cholesterol levels in the combined training group compared to the control group.

Conclusion

Combined training, as part of cardiac rehabilitation in patients performing coronary artery bypass graft by effecting on ABCB4 gene expression, involved in fat metabolism, improves reverse cholesterol transfer process.

کلیدواژه‌ها English

Coronary artery bypass graft
Combined training
ABCB4 gene
1. Gelissen IC, Harris M, Rye KA, Quinn C, Brown AJ, Kockx M, et al. ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I. Arterioscler Thromb Vasc Biol 2006; 26(3): 534-40.
2. Le Goff W, Settle M, Greene DJ, Morton RE, Smith JD. Reevaluationof the role of the multidrug-resistant P-glycoprotein in cellularcholesterol homeostasis. J Lipid Res 2006; 47:51–8.
3. Geuken E, Visser DS, Leuvenink HGD, De Jong KP, Peeters PMJG, Slooff MJH, Kuipers F, Porte RJ (2005). Hepatic Expression of ABC Transporters G5 and G8 Does Not Correlate with Biliary Cholesterol Secretion in Liver Transplant Patients Hepatol. 42:1166-1174.
4. Smit JJM, Schinkel AH, Oude Elferink RPJ, Groen AK, Wagenaar E, et al. Homozygous disruption of the murine mdr2 P-glycoprotein geneleads to a complete absence of phospholipid from bile and to liverdisease. Cell 1993; 75:451–62.
5. Hoang A, Tefft C, Duffy SJ, Formosa M, Henstridge DC, Kingwell BA et al. (2008). ABCA1 expression in humans is associated with physical activity and alcohol consumption. Atherosclerosis; 197(1): 197-203.
6. Fatone C, Guescini M, Balducci S, Battistoni S, Settequattrini A, Pippi R, et al. Two weekly sessions ofcombined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetesmellitus and metabolic syndrome. J Endocrinol Invest 2010;33(7):489-95.
7. Ghroubi S, et al. (2013) Effects of a low intensity dynamic-resistance training protocol using an isokinetic dynamometer on muscular strength and aerobic capacity after coronary artery bypass grafting. Ann Phys Rehabil Med 56)2), 85-101. [Persian]
8. Arthur HM, Gunn E, Thorpe KE, Ginis KM, Mataseje L, McCartney N, et al. Effect of aerobic vs combinedaerobic-strength training on 1-year, post-cardiac rehabilitation outcomes in women after a cardiac event. J RehabilMed 2008;39(9):730-5.
9. Gaiini Abas Ali, Satari Fard Sadegh, Kafi Zade Sara, Nejatian Mostafa 2013. Comparison of eight weeks of combined and aerobic training on functional capacity, body composition and strength of heart patients after coronary artery bypass surgery. The journal of cardiovascular nursing, period 2(1): 34-41. [Persian]
10. Rashidlamir A. Investigation of the Effect of Aerobic and Resistance Exercises on Peripheral BloodMononuclear Cells ABCG1 Gene Expression in Female Athletes. Journal of Shahid Sadoughi University of MedicalSciences 2012;20(1):1-9. [Persian]
11. Gelissen IC, Harris M, Rye KA, Quinn C, Brown AJ, Kockx M, et al. ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I. Arterioscler Thromb Vasc Biol 2006; 26(3): 534-40
12. Butcher, L. R., Thomas, A., Backx, K., Roberts, A. L. E. D., Webb, R., & Morris, K. (2008). Low-intensity exercise exerts beneficial effects on plasma lipids via PPARgamma. Medicine and science in sports and exercise, 40(7), 1263-1270.
13. Srivastava N. (2002). ATP binding cassette transporter A1 – key roles in cellular lipid transport and atherosclerosis. Mol Cell Biochem; 237:155-64
14. Mahdiniya M. New findings in prevention and identification of risk factors of cardiovascula attaks. Proceedings of Cardiovascular Nursing Continuing Education.Tehran.2006; p: 172. [Persian]
15. Oude Elferink RP, Paulusma CC: Function and pathophysiological importance of ABCB4 (MDR3 P-glycoprotein). Pflugers Arch. 453, 601–610 (2007). [Persian
16. Tall, A. R., Jiang, X. C., Luo, Y., & Silver, D. (2000). George Lyman Duff Memorial Lecture Lipid Transfer Proteins, HDL Metabolism, and Atherogenesis. Arteriosclerosis, thrombosis, and vascular biology, 20(5), 1185-1188
17. Talebi-Garakani E, safarzade A. (2013). The Effect of Resistance Training Intensity on Serum ApoA-I Concentration in Streptozotocin-Induced Diabetic Rats. Iranian Journal of Endocrinology and Metabolism.; 15(2):183-9. [Persian]
18. Jafari, M., Leaf, D. A., MacRae, H., Kasem, J., O'Conner, P., Pullinger, C., ... & Kane, J. P. (2003). The effects of physical exercise on plasma prebeta-1 high-density lipoprotein. Metabolism, 52(4), 437-442.
19. Rashidlamir, A., Ghanbari-Niaki, A., Saadatnia, A., (2011). The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-I. World Journal of Sport Sciences, 4(2): 144-150. [Persian]
20. Mashaykhi NR, Sadrneya S, Acherei A, Javaheri J, ahmadlou m.(2013). The correlation between serum Apo lipoprotein A1 and Apo lipoprotein B with coronary artery disease and its severity. Arak University of Medical Sciences Journal.; 16(6):82-9. [Persian]
21. Khabazian BM, Ghanbari-Niaki A, reza Safarzadeh-Golpordesari A, Ebrahimi M, Rahbarizadeh F, Abednazari H. (2009). Endurance training enhances ABCA1 expression in rat small intestine. European journal of applied physiology.;107(3):351-8. [Persian]
22. Langheim S, Yu L, von Bergmann K, Lutjohann D, Xu F, Hobbs HH, et al. ABCG5 and ABCG8 require MDR2 for secretion of cholesterolinto bile. J Lipid Res 2005; 46:1732–8.
23. Memari Moghadam M, Talebi Gorgani2010.Comparison of total antioxidant capacity, oxidative stress status and lipoprotein of cycling athlete with non athletes. Sports and motor life sciences: 2(4): 19-26.
24. Krieger M. (2001). Scavenger receptor class B type I is a multiligand HDL receptor that influences diverse physiologic systems. J Clin Invest 108: 793-7.
25. Rashid lamir A, Droodi S, Ebrahimie Atri A. (2012). The investigation effect of aerobic and resistance exercise on lymphocytes ABCA1 gene expression in well-trained girls. Journal of Sport and Biomotor Science; 1(12):5-22.
26. Bruno Bavaresco Gambassi1, Fabiano de Jesus Furtado Almeida, Ana Eugênia Araújo Furtado Almeida1, Daniela Alves Flexa Ribeiro, Rômulo Sérgio Araújo, Luiz Filipe Costa Chaves. Acute Response to Aerobic Exercise on Autonomic Cardiac Control of Patients in Phase III of Cardiovascular Rehabilitation Program Following Coronary Artery Bypass Grafting. Brazilian Journal of Cardiovascular Surgery. vol.34 no.3 São José do Rio Preto May/June 2019 Epub July 22, 2019. ISSN 1678-9741.
27. Amir Rashidlamir, Mostafa Dastani, Arash Saadatnia, Mohammad Reza Bassami. Effect of Cardiac Rehabilitation Training on ABCA1 Expression in Lymphocytes of Patients Undergoing Coronary Artery Bypass Graft Operation. Zahedan Journal of Research in Medical Sciences: 2018; 20 (6); e11277. [Persian]
28. Hajighsemi AR’ Ravasi AA’Kordi MR. Rashidlamir A’ Ghorghi investigation of the Effect of Cardiac Rehabiood Monoucler Cells ABCA1 Gene Exprssion in Myocardial infractions patient .2016; Jurnal of knowledge & Healeth. 2017 :11.4;23-29. [Persian]
29. Jafari Mahtab, Leaf D.A, MacRae H, et al. Kane. 2003. “The Effects of Physical Exercise on Plasma Pre beta-1 High-Density Lipoprotein”. Metabolism.52 (4): 437-442. [Persian].
30. Asad Mohamad Reza 2012.The effect of eight weeks of aerobic, resistance and combined exercise on HDL, LDL cholesterol and cardiorespiratory fitness in obese men. Applied research of management and life sciences in sports. 3: 57-64. [Persian]
31. Habib Zade Nasim, Rahmani Nia Farhad 2010. Investigating the effect of two months of walking exercise on serum lipids and body mass index on obese girls. The journal of Mashhad university of medical sciences.
32. Hoang A, Tefft C, Duffy SJ, Formosa M, Henstridge DC, Kingwell BA et al. (2008). ABCA1 expression in humans is associated with physical activity and alcohol consumption. Atherosclerosis; 197(1): 197-203.
33. Gonzales, E., A. Davit-Spraul, C. Baussan, C. Buffet, M. Maurice, and E. Jacquemin, 2009, Liver diseases related to MDR3 (ABCB4). gene defi ciency. Front. Biosci. 14: 4242 – 4256.
34. The effect of combined training on lipid profile of white blood cells ABCB4 gene expression
and HDL to LDL ratio in middle- aged men after coronary artery bypass graft.
35. Pennings M, Hildebrand RB, Ye D, Kunne C, Van Berkel TJ, Groen AK, Van Eck M. Bone marrow-derived multidrug resistance protein ABCB4 protects against atherosclerotic lesion development in LDL receptor knockout mice. Cardiovascular research. 2007 Oct 1;76(1):175-83.
36. Kawaguchi K, Morita M. ABC transporter subfamily D: distinct differences in behavior between ABCD1–3 and ABCD4 in subcellular localization, function, and human disease. BioMed research international. 2016 Sep 28;2016.
37. Fernandez ML, Webb D. The LDL to HDL cholesterol ratio as a valuable tool to evaluate coronary heart disease risk. Journal of the American College of Nutrition. 2008 Feb 1;27(1):1-5.
38. Panagiotakos DB, Toutouzas PK. Importance of LDL/HDL cholesterol ratio as a predictor for coronary heart disease events in patients with heterozygous familial hypercholesterolaemia: a 15-year follow-up (1987-2002). Current medical research and opinion. 2003 Jan 1;19(2):89-94.