اثر محافظتی تمرین هوازی همراه با مکمل کپسایسین بر شاخص‌های آپوپتوز قلب موش‌های صحرایی تغذیه شده با رژیم غذایی پرچرب

نویسندگان
مریم داوران 1
احمد عبدی 1
آسیه عباسی دلویی 1
جواد مهربانی 2
1 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 گروه فیزیولوژی ورزشی، دانشگاه گیلان، رشت، ایران
چکیده
مقدمه

مصرف رژیم غذایی پر چرب باعث چاقی ناشی از رژیم غذایی شده و با چندین خطر-قلبی عروقی از جمله سندرم متابولیکی و آپوپتوز همراه است. هدف از پژوهش حاضر بررسی اثر تمرین هوازی همراه با کپسایسین بر تنظیم کننده آپوپتوزی BAX (Bax)، لنفوم سلول B2 (Bcl2) و نسبت Bax/BCL2 قلبی در موش‌های تغذیه شده با رژیم غذایی پرچرب (HFD) می‌باشد.

مواد و روش‌­ها

40 سر موش صحرایی نر ویستار به مدت 8 هفته با رژیم غذایی نرمال (8=n، ND) و HFD (32=n) تغذیه شدند. سپس همه موش‌ها به 5 گروه: ND، HFD، رژیم غذایی پرچرب-تمرین (HFDT)، رژیم غذایی پرچرب-کپسایسین (HFDCap) و رژیم غذایی پرچرب-تمرین- کپسایسین (HFDTCap) تقسیم شدند. گروه‌های تمرین به مدت هشت هفته برنامه تمرین هوازی فزاینده (25-15 متر در دقیقه، 60-30 دقیقه در روز، پنج روز هفته) را روی تردمیل انجام دادند. کپسایسین (mg/kg/day 4) یک بار در روز به صورت خوراکی با گاواژ خورانده شد. میزان Bax، Bcl2 بافت قلب به روش الایزا اندازه‌گیری شد.

یافته­‌ها

نتایج پژوهش حاضر نشان‌دهنده کاهش Bax و نسبت Bax/Bcl2 در گروه‌های HFDT، HFDCap و HFDTCap نسبت به HFD بود (05/0p<). Bax و نسبت Bax/Bcl2 در HFDTCap نسبت به گروه‌های HFDT و HFDCap کاهش معنی‌داری داشت (05/0p<). همچنین Bcl2 در گروه‌های HFDT، HFDCap و HFDTCap نسبت به HFD افزایش معنی‌داری داشت (001/0p<).

نتیجه­‌گیری

تمرین هوازی و مصرف کپسایسین باعث بهبود آپوپتوز ناشی از چاقی می‌شود، و پیشنهاد می‌شود فعالیت ورزشی و کپسایسین به عنوان یک روش مناسب برای درمان این شرایط بالینی در نظر گرفته شود.
کلیدواژه‌ها
فعالیت ورزشی
کپسایسین
Bax
Bcl2
آپوپتوز
چاقی

عنوان مقاله English

Protective Effect of Aerobic Training Along with Capsaicin Supplementation on Factors of of Cardiac Apoptosis in Rat Fed a High-Fat Diet

نویسندگان English

Maryam Davaran 1
Aahmad Abdi 1
Asieh Abbassi Daloii 1
Javad Mehrabani 2
1 Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, amol, I. R. Iran
2 Department of Exercise Physiology, Gilan Univercity, Rasht, Iran
چکیده English

Introduction

High-fat diet intake leads to diet-induced obesity in concert with multiple cardiovascular risk factors such as metabolic syndrome and apoptosis. The aim of this study was to evaluate the effects of aerobic training with along capsaicin on cardiac apoptosis regulator BAX (Bax), B- cell lymphoma 2 (Bcl2) and Bax/Bcl2 in rats fed a high-fat diet (HFD).

Materials and Methods

40 male Wistar rats were fed a normal diet (ND, n = 8) or HFD (n = 32) for 8 weeks. After 8 weeks, all rats were divided into 5 groups: ND, HFD, high-fat diet-training (HFDT), high-fat diet-capsaicin (HFDCap), high-fat diet-training-capsaicin (HFDTCap). Training groups have performed a progressive aerobic running program (at 15-25 m/min, 30-60 min/day, and 5 days/week) on a motor-driven treadmill for eight weeks. Capsaicin (4 mg/kg/day) was administered orally, by gavage, once a day.

Results

The results of this study showed that Bax and Bax/Bcl2 in HFDT (Respectively p=0.016, p=0.000), HFDCap (Respectively p=0.014, p=0.000) and HFDTCap (Respectively p=0.000, p=0.000) groups was significantly decreased compared to HFD. Bax and Bax/Bcl2 in HFDTCap was significantly decreased compared to HFDT (Respectively p=0.034, p=0.033) and HFDCap (Respectively p=0.038, p=0.036) groups. Also, Bcl2 significantly increased in to HFDT (p=0.001) and HFDCap (p=0.000) and HFDTCap (p=0.000) groups compared to HFD.

Conclusion

Exercise and capsaicin improve the apoptosis induced by obesity and suggest that exercise and capsaicin can be considered as an excellent candidate for therapy of these clinical conditions.

کلیدواژه‌ها English

Exercise
Capsaicin
Bax
Bcl2
Apoptosis
obesity
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