Analysis of Chemical Compounds in Extracts of Uritica dioica L., Mentha longifolia L., and Tragopogon collinus Collected from the Natural Habitats of Meshgin Shahr Region and Comparative Therapeutic Effects of Their Combination with Ciprofloxacin on Common Bacterial Urinary Tract Infections

Authors
Hojjat Eghbal 1
Nima Mohammadnejad khiavi 2
ali shahi garahlar 3
neda jahani 4
1 Department of Phytochemistry, Basic Science Research Center, Tabriz University, Tabriz, Iran
2 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Department of Plant Sciences and Medicinal Plants, Faculty of Meshgin Shahr Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
4 Department of Dentistry, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Introduction

Considering the antibacterial effects of medicinal plants and the side effects of chemical drugs, replacing chemical drugs with herbal drugs is of particular importance. Therefore, this study aims to investigate the chemical compounds in the extracts of Uritica dioica L., Mentha longifolia L., and Tragopogon collinus collected from the vegetative regions of Meshgin Shahr and compare the therapeutic effect of their combination with that of ciprofloxacin against common bacteria of urinary tract infection.

Materials and Methods

The main phenolic compounds of the desired medicinal plants were identified using HPLC after collecting from the original habitat and extraction by the maceration method. Then, the antimicrobial properties of the combination of extracts against common urinary tract infection bacteria were investigated and compared with those of the ciprofloxacin drug using the disk diffusion method. In addition, the MIC values of the combination of extracts at concentrations of 12.5, 25, 50, and 100 mg/ml against the studied bacteria were determined in 3 replicates.

Results

The main phenolic compounds identified from the hydroalcoholic extract of Uritica dioica L,. including catechin and rutin, Mentha longifolia L., including gallic acid and quercetin, and Tragopogon collinus including ursolic acid and caffeic acid. According to the obtained results, it was found that the mixture of plant extracts shows relatively similar antimicrobial properties to ciprofloxacin.

Conclusion

According to the obtained results, it can be said that the mixture of extract of Uritica dioica L., Mentha longifolia L., and Tragopogon collinus medicinal plants by conducting more studies, especially at the in vivo conditions, can be a suitable product for treatment of urinary tract infection.
Keywords
Mentha longifolia L
Antimicrobial property
Herbal extract
Urinary tract infection
Uritica dioica L
1. Nwanze PI, Nwaru LM, Oranusi SU, Dimkpa U, Okwu MU, Babatunde BB, Anake TA, Jatto W, Asagwara CE. Urinary tract infection in Okada village: Prevalence and antimicrobial susceptibility pattern. Scientific Research and Essay. 2007;2(4):112-6.
2. Barnett BJ, Stephens DS. Urinary tract infection: an overview. The American journal of the medical sciences. 1997 Oct 1;314(4):245-9.
3. Whiting P, Westwood M, Watt I, Cooper J, Kleijnen J. Rapid tests and urine sampling techniques for the diagnosis of urinary tract infection (UTI) in children under five years: a systematic review. BMC pediatrics. 2005 Dec;5(1):1-3.
4. Khatony A, Mohammadi M, Jafari F, Vaisi-Raygani A, Jalali R, Salari N. Urinary tract infection among Iranian older adults: a systematic review and meta-analysis. Ageing International. 2021 Jun;46:170-81.
5. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food control. 2007 May 1;18(5):414-20.
6. Bonjar S. Evaluation of antibacterial properties of some medicinal plants used in Iran. Journal of ethnopharmacology. 2004 Oct 1;94(2-3):301-5.
7. Joshi BC, Mukhija M, Kalia AN. Pharmacognostical review of Urtica dioica L. International Journal of Green Pharmacy (IJGP). 2014;8(4).
8. Farhan SA, Faraj M, Al-Shemari HH, Jassim AM. Study of some Urtica dioica L. Leaves components and effect of their extracts on growth of pathogenic bacteria and identify of some flavonoids by HPLC. Al-Mustansiriya J. Sci. 2012;23(3):79-86.
9. Farzami B, Ahmadvand D, Vardasbi S, Majin FJ, Khaghani SH. Induction of insulin secretion by a component of Urtica dioica leave extract in perifused Islets of Langerhans and its in vivo effects in normal and streptozotocin diabetic rats. Journal of ethnopharmacology. 2003 Nov 1;89(1):47-53.
10. Buso P, Manfredini S, Reza Ahmadi-Ashtiani H, Sciabica S, Buzzi R, Vertuani S, Baldisserotto A. Iranian medicinal plants: from ethnomedicine to actual studies. Medicina. 2020 Feb 26;56(3):97.
11. Dolatkhahi M, Ghorbani Nohooji M, Mehrafarin A, Amini Nejad GR, Dolatkhahi A. Ethnobotanical study of medicinal plants in Kazeroon, Iran: Identification, distribution and traditional usage. Journal of medicinal plants. 2012 May 10;11(42):163-78.
12. Gulluce M, Sahin F, Sokmen MÜ, Ozer H, Daferera D, Sokmen AT, Polissiou M, Adiguzel AY, Ozkan Hİ. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food chemistry. 2007 Jan 1;103(4):1449-56.
13. Abolfathi11 E, Amirmozafari N, Ghaemi E. Antimicrobial activity of Ethanolic extracts of (Tragopogon collinus L. and Allium eriophyllum L.) on some selected environmental and pathogenic bacteria. Biotechnological Journal of Environmental Microbiology. 2022 Apr 1;1(1).
14. Gadisa E, Tadesse E. Antimicrobial activity of medicinal plants used for urinary tract infections in pastoralist community in Ethiopia. BMC Complementary Medicine and Therapies. 2021 Dec;21:1-9.
15. Acharjee M, Zerin N, Ishma T, Mahmud MR. In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics. New Microbes and New Infections. 2023 Jan 1;51:101076.
16. Thakur R, Jain N, Pathak R, Sandhu SS. Practices in wound healing studies of plants. Evidence-based complementary and alternative medicine. 2011 Jan 1;2011.
17. Azizi Alidoust F, Anvari M, Atayi Jaliseh S. Antimicrobial Activity of Aqueous and Alcoholic Extracts of Chamomile, Fleawort, Aquatic Pennyroyal and Nettle Plants on Klebsiella pneumoniae and Comparing Their Effects with Common Antibiotics. Iranian Journal of Medical Microbiology. 2020 Jul 10;14(4):361-73.
18. Hanberger, H., Walther, S., Leone, M., Barie, P. S., Rello, J., and Lipman, J. 2011. Increased mortality associated with methicillin -resistant Staphylococcus aureus (MRSA) infection in the Intensive Care unit: results from the EPIC II study. International Journal of Antimicrobial Agents. 38(4): 331 - 335.
19. Negi, P. S. 2012. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology. 156(1): 7 -17.
20. Hasheminya, S. M., and Dehghannya, J. 2020. Composition, phenolic content, antioxidant and antimicrobial activity of Pistacia atlantica subsp. kurdica hulls’ essential oil. Food Bioscience. 34: 100510.
21. Haghighi, M., Sharif Rohani, M., Pourmoghim, H., Samadi, M., Tavoli, M., Eslami, M., and Yusefi, R. 2017. Enhancement of immune responses of rainbow trout (Oncorhynchus mykis) fed a diet supplemented with Aloe vera extract. Iranian Journal of Fisheries Sciences. 16(3): 884 -896.
22. Hasheminya, S. M., and Dehghannya, J. 2020. Green synthesis and characterization of copper nanoparticles using Eryngium caucasicum Trautv aqueous extracts and its antioxidant and antimicrobial properties. Particular Science and Technology. 38(8): 1019 -1026
23. Klančnik A, Piskernik S, Jeršek B, Možina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. Journal of microbiological methods. 2010 May 1;81(2):121-6.
24. Falsafi S, Tayebi Z, Msoumi M, Mirzaei A. Antimicrobial susceptibility pattern of human pathogenic bacteria isolated from patients with urinary tract infection. New Cellular and Molecular Biotechnology Journal. 2014 Sep 10;4(15):83-9.
25. Saraj M S, Mowla K, Ghorbani A, Etemadi A, Cheraghy M, Mahmoodlo A et al . Identification of Outpatient Urinary Pathogens and Antibiotic Susceptibility Pattern in Ahwaz, Iran 2002-2003. yafte 2005; 6 (4) :41-47
26. Mahmoudi H, Alikhani MY, Arabestani M, Khosravi S. Evaluation prevalence agents of urinary tract infection and antibiotic resistance in patients admitted to hospitals in Hamadan University of Medical Sciences 1391-92. Pajouhan Scientific Journal. 2014 Jun 10;12(3):20-7.
27. Rahimi M K, Falsafi S, Tayebi Z, Msoumi M, RezaFerasat pour M R, Mirzaei A. Antimicrobial susceptibility pattern of human pathogenic bacteria isolated from patients with urinary tract infection. NCMBJ 2014; 4 (15) :83-89.
28. Ushimaru PI, Silva MT, Di Stasi LC, Barbosa L, Fernandes Junior A. Antibacterial activity of medicinal plant extracts. Brazilian Journal of Microbiology. 2007;38:717-9.
29. Gulchin İ, Küfrevioğlu İ, Oktay M, BüyükokuroğluME.Antioxidant, antimicrobial antiulcer and analgestic activities of nettle (Urtica dioica L.), J. Ethnopharmacol.2004; 90: 205 - 15. 16.
30. Kukrić ZZ, Topalić-Trivunović LN, Kukavica BM, Matoš SB, Pavičić SS, Boroja MM, Savić AV. Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.). Acta periodica technologica. 2012(43):257-72.
31. Shahidi F, Tabatabai Yazdi, F, Alizadeh Behbahani B, Roshanak S, Norouzi N, Vasiei A. Antibacterial effect of Sheng plant extract on Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli and Salmonella typhi in laboratory conditions. Infectious and tropical diseases of Iran. 2018;24(84):1-10. [persian]
32. Ghaffari M, Mahdavi Ortakand M, Baghbani Arani F. Studying the antimicrobial and synergistic effect of peppermint essential oil (Mentha pulegium L.) and ciprofloxacin antibiotic in Escherichia coli strains isolated from urinary tract infection. Biological knowledge of Iran. 2014: 10 (4): 31-39.
33. Hosseingalizadeh E, Elmi S, Razavi S and Zarini G. Antimicrobial effect of oregano plant extracts on Escherichia coli urinary isolates, 19th National Congress and 7th International Congress of Biology of Iran. 2015.