Antifungul activitiy of endophytes isolated from Rhabdosciadium aucheri against some human and plant pathogenic fungi

Authors
Hanieh Sarfarazi Saleh 1
Reza Habibipour 1
Moshen Rajabi 2
1 Department of Microbiology, Faculty of Medical Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran
2 Forest and Pasture Research Department, Hamadan Province Agriculture and Natural Resources Research and Education Center, Agriculture Research, Education and Promotion Organization, Hamadan, Iran
Abstract


Introduction

Rhabdosciadium aucheri Boiss belongs to the Apiaceae family, and is an exclusive species of Iran that grows in Hamedan, Lorestan, Chaharmahal Bakhtiari, Kohkiluyeh, Boyer-Ahmad, and Tehran provinces. Therefore, the aim of this study was to investigate the effect of endophytic fungi isolated from R. aucheri on human and plant pathogenic fungi.

Materials and Methods

In this observational and laboratory study, fungi were cultured on a PDA medium. The effect of endophytic fungi isolated from R. aucheri (Alternaria rosae K11, Alternaria radicina K2, Alternaria radicina K18, A. radicina K21, and Paraphoma radicana K23) on human (Cryptococcus neoformans, Candida albicans, Candida glabrata, Aspergillus niger, Aspergillus flavus and Pseudallescheria boydii) and plant (Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum) pathogenic fungi was investigated by the cross-culture method in the form of randomized complete block design with three repetitions. The obtained results were analyzed using SPSS software and compared with Duncan's test.

Results

The results of the present study showed that the endophytes isolated from R. aucheri have significant antimicrobial effects against some plant and human pathogenic fungi (P<0.01), so that Alternaria radicina 2(K21) and Alternaria rosae K11 had the highest antifungal activity against Candida glabrata and Aspergillus flavus, respectively. The effect of Alternaria radicina against Fusarium oxysporum with 15% showed the growth rate of this endophyte against plant pathogenic fungi. In addition for the the effect of A. radicina 2(K2) against Rhizoctonia solani, the inhibition percentage was 70%.

Conclusion

The present study showed that endophytes isolated from the R. aucheri plant have potential antimicrobial effects against some plant and human pathogenic fungi. However, investigating the inhibitory effects of these endophytes requires further research.

Keywords: Endophyte, Secondary metabolite, Fungi, Rhabdosciadium aucheri, Microorganism

Keywords
Endophyte
Secondary metabolite
Fungi
Rhabdosciadium aucheri
Microorganism
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