Phenotypic evaluation of the prevalence of efflux pumps and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa and staphylococcus aureus among burned patients

Authors
fateme fahami
teena dadgar
hamidreza pordeli
Abstract
Abstract

Introduction: The most common microorganisms that cause hospital infections in patients with burnsare Pseudomonas aeruginosa and Staphylococcus aureus. Efflux pumps play a very important role in creating antibiotic resistance of Staphylococcus aureus and Pseudomonas aeruginosa strains. This study aimed to investigate microbial resistance and phenotypic investigation of the efflux pump in Pseudomonas aeruginosa and Staphylococcus aureus strains isolated from patients with burns.

Materials and methods: In this descriptive and cross-sectional study,45 swab samples were collected from the wounds of 2nd and 3rd degree burn patients at the Imam Ali (A.S.) Medical Center, burn department in Bojnord city over a period of 6 months. Biochemical tests were performed. The drug sensitivity pattern was done by the disk diffusion method, phenotypic tests, and acid test to detect the production of broad-spectrum beta-lactamase. Phenotypic investigation of efflux pump activity was done using the Cardwell method. Results: In the present study,20 wound samples of burn patients were infected with Pseudomonas aeruginosa and 10 samples were infected with Staphylococcus aureus. Staphylococcus aureus was resistant to ampicillin, penicillin and cefazolin 100% and cefotaxime, levofloxacin, and ciprofloxacin, 70%, 80%, 90%, respectively, and Pseudomonas aeruginosa was resistant to imipenem, ciprofloxacin, levofloxacin, and ceftazidime, 85%, respectively. 65%, 55%, 85% were resistant. 60% Pseudomonas aeruginosa produced ESBL, 70% Staphylococcus aureus produced beta-lactamase. 75% and 70%. MDR isolates of Pseudomonas aeruginosa and Staphylococcus aureus had efflux pumps.

Conclusion: The results showed that in the phenotypic more than 70% of MDR isolates of Pseudomonas aeruginosa and Staphylococcus aureus have an efflux pump, so it is important to check the presence of this pump in proposing a suitable treatment model for patients infected with these bacteria.




Keywords
Keywords: antibiotic resistance
Pseudomonas aeruginosa
Staphylococcus Aureus
efflux pump
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