Prevalence of Pathogenic Genes ureA and vacA of Helicobacter pylori Isolated in Patients with gastric ulcer

Authors
aliakbar jannatabadi
mahnaz divandari
MOHSEN MOHAMMADI
mitra mehrkhah
Abstract

Introduction: Helicobacter pylori infection occurs in more than half of the human population. Helicobacter pylori is one of the main causes of gastric ulcers. The aim of the present study was to correlate the molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on the analysis of vacuolating toxin (vacA) and urease A (ureA) genes, with the clinical and histological findings in patients with H. pylori-associated gastric ulcer.

Methods: A total of 95 patients (54 women and 41 men) with gastric ulcer from Sabzevar city were included in the study. Gastric tissue biopsies were collected, and the vacA and ureA genotypes were investigated by polymerase chain reaction (PCR). In this study, the rapid urease test (RUT) was also used to check Helicobacter infection.

Results: Out of a total of 95 gastric ulcer samples, 45.3% were found to be infected with Helicobacter pylori. The presence of the ureA gene was reported in 28.4% of the cases, while the frequency of the vacA gene was 34.7%. In this study, the urease test was positive in 22.1% of the samples.

Conclusion: Based on the chi-square test and data analysis, no significant relationship was observed between Helicobacter infection and the presence of genes in relation to gender. However, there was a significant relationship between the presence of the vacA and ureA genes and Helicobacter pylori infection.

Keywords
Helicobacter pylori
gastric ulcers
vacA
ureA
PCR
Urease test
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