بررسی ارتباط بیان ژن‌های miR-125-b و miR-455-3p با شدت علائم بالینی بیماری آلزایمر

نویسندگان
محمدرضا خاکزاد
مریم گوهری
فائزه باستان
علیرضا آل هاشمی
مهران همام
طیبه ربانی نیا
دانشگاه آزاد اسلامی-واحدعلوم پزشکی مشهد
چکیده
مقدمه: بیماری ‌آلزایمر شایع‌ترین نوع دمانس است که روش‌های غیرتهاجمی برای تشخیص زودرس آن وجود ندارد. در سال‌های اخیر در چندین مطالعه نقش miR-125 و miR-455 را به ‌عنوان عامل موثر بر ژن‌های مرتبط با پاتوژنز بیماری ‌آلزایمر مطرح شده است. در این مطالعه ارتباط بیان ژن این دو miRNA با شدت علائم بالینی بیماری‌ آلزایمر بررسی‌شد.

روش:50 بیمار مبتلا به آلزایمر براساس معیارهای DSM-5 شناسایی و شدت بیماری با پرسشنامه کوتاه وضعیت ذهنی(MMSE) و مقیاس جهانی ارزیابی دمانس رولاندس (RUDAS) ارزیابی شد. از بیماران خون وریدی گرفته شد و سلول‌های منونوکلئارخون‌محیطی جدا شدند. با استفاده از کیت اختصاصی MicroRNAsها استخراج، از آن‌ها cDNA سنتز و با روش RT-PCR شدت ‌بیان miR-125 و miR-455 تعیین‌شد.

یافته‎ها:50 بیمار با میانگین سنی بیماران 7/73±76/00سال شامل 54 درصد مرد بررسی شدند. میانگین فراوانی بیان ژن‌های miR-125 و miR-455 به ترتیب 2/84±3/68 و 4/69±5/89 بود که نسبت به جمعیت عادی شدت بیشتری داشته است (0/05>P). بین شدت بیان ژن‌ها با شدت بیماری ارتباط معناداری مشاهده نشد. سن، جنسیت و طول مدت بیماری ابتلا به بیماری نیز ارتباطی با شدت بیان ژن نداشت.

نتیجه‎گیری: بیان ژن‌های miR-125 و miR-455 در بیماران مبتلا به آلزایمر به‌طور معناداری بیشتر است؛ اما با شدت بیماری ارتباطی نداشت. پیشنهاد می‌شود در آینده مارکرهای مرتبط با این مولکول‌‎ها در مسیرهای درون سلولی بررسی شوند.
کلیدواژه‌ها
بیماری آلزایمر
MMSE
miR-125 و miR-455
بیان ژن

عنوان مقاله English

Investigating the Relationship between miR-125-b and miR-455-3p Gene Expression and the Severity of Alzheimer's Disease

نویسندگان English

Mohammad reza Khakzad
Maryam Gohari
Faezeh Bastan
Ali reza Al-Hashemi
Mehran Homam
Tayebeh Rabani nia
Islamic Azad University, Mashhad, Iran.
چکیده English

Introduction: Alzheimer's disease is the most common type of dementia, and as of now, there has not been a non-invasive method known for its preclinical diagnosis. In recent years, there have been several studies highlighting the role of miR-125 and miR-455 as effective factors in genes related to the pathogenesis of Alzheimer's disease. In this study, the relationship between gene expression levels of these two miRNAs and the severity of Alzheimer's clinical symptoms in patients with Alzheimer's disease was investigated.

Methods: 50 Patients with Alzheimer's were identified according to the DSM-5 criteria and the severity of the disease was evaluated with the Mini-Mental State Examination (MMSE) and the Roland Dementia Assessment Scale (RUDAS). Venous blood was drawn from the patients/ Venous blood samples were collected from the patients and peripheral blood mononuclear cells were isolated. MicroRNAs were extracted using a specific kit, cDNA was synthesized, and the expression levels of miR-125 and miR-455 were measured by using the PCR-RT method.

Results: Patients had a mean age of 76.00±7.73 years, including 54% men, were studied. A mean frequency of 3.68±2.84 and 5.89±4.69 was calculated for gene expression levels of miR-125 and miR-455, respectively, which was significantly higher than that of the control population (P<0.05). Despite that, there was no significant correlation between the gene expression levels and the severity of the disease. In addition, age, gender, and the duration of the disease were not associated with the gene expression levels analyzed in this study.

Conclusion: The expression of miR-125 and miR-455 genes is significantly higher in patients with Alzheimer's disease; however, it was not related to the severity of the disease. It is suggested that future studies focus more on the markers related to these molecules in intracellular pathways.

کلیدواژه‌ها English

Alzheimer's disease
MMSE
miR-125 and miR-455
gene expression
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