تاثیر هشت هفته تمرین مقاومتی و استقامتی شدید و متوسط بر بیان mir-133a، Runx2 و PPARγ در مغز استخوان در موش‌های صحرایی نر سالمند نژاد ویستار

نویسندگان
زهرا همتی فارسانی
ابراهیم بنی طالبی
محمد فرامرزی
امین بی غم صادق
دانشگاه شهرکرد
چکیده
مقدمه

بهترین راه پیشگیری از بروز پوکی استخوان در سالمندی جلوگیری از کاهش تراکم استخوانی و یا تلاش در جهت حفظ آن در سنین جوانی است. هدف از مطالعه حاضر، تاثیر شدت‌‌های مختلف تمرین استقامتی و مقاومتی بر بیان microRNA-133a (mir-133a)، Runx2 و PPARγ در مغز استخوان ران موش‌‌های صحرایی نر سالمند بود.

مواد و روش‌‌ها

این مطالعه تجربی، روی 40 سر موش‌‌های صحرایی نر بالغ از نژاد ویستار انجام شد. موش‌‌های صحرایی در 5 گروه مساوی 8 تایی شامل تمرینات مقاومتی و استقامتی با شدت متوسط و شدید و گروه کنترل قرار گرفتند. گروه‌‌های تمرینی، 5 روز در هفته به مدت 8 هفته برنامه تمرینی را طبق پروتکل مقاومتی یا استقامتی اجرا کردند. بر روی موش‌‌های صحرایی گروه کنترل، هیچگونه مداخله‌‌ای صورت نگرفت. بعد از دوره تمرین بیان mir-133a، Runx2 و PPARγ به روش RT–PCR اندازه‌‌گیری شدند. تجزیه تحلیل آماری با استفاده از آزمون کروسکال والیس با سطح معنی‌‌داری 0/05>P انجام شد.

یافته‌‌ها

این پژوهش نشان داد بیان mir-133a (0/701P=)، Runx2 (P=0/948) و PPARγ (P=0/364) در گروه تمرین با شدت بالا و شدت متوسط و گروه کنترل تفاوت معنی‌‌داری نداشت.

نتیجه‌‌گیری

به نظر می‌‌رسد تغییرات سلولی متابولیسم استخوان پس از انجام چنین تمریناتی نیاز به دوره‌‌های طولانی‌‌تر تمرین ورزشی دارد که باید در تحقیقات آینده مورد بررسی قرار بگیرد.
کلیدواژه‌ها
mir-133a
RUNX2
PPARγ
استخوان ران
تمرین استقامتی و مقاومتی
متابولیسم استخوان

عنوان مقاله English

The effect of Eight Weeks of Moderate and High Intensity endurance and Resistance Training on expression mir-133a، Runx2 and PPARγ on femor bone marrow in Older male Wistar rats

نویسندگان English

zahra hemati farsani
Ebrahim banitalebi
Mohamad Faramarzi
Amin Bigham-Sadegh
Shahrekord University
چکیده English

Objective: The best way to prevent osteoporosis, at old age is to prevent bone loss and at young age is trying to keep bones healthy, therefore The aim of this study was to determine the effects of compare effects of aerobic and resistance exercise training protocols with different intensities on microRNA-133a (mir-133a), Runx2 and Peroxisome proliferator-activated receptors-gamma (PPARγ) in femor bone marrow Wistar elderly rats.. Method: This experimental study was done on 40 Wistar male rats . They were divided into 5 equal groups (n=8) incloud moderate or high intensity resistance training, moderate or high intensity endurance training and control. The two training groups completed 8 weeks of training program, 5 days a week according to protocol. After completing training, expression of mir-133a, Runx2 and PPARγ were measured RT-PCR. The statistical analysis was performed using Kruskal-Wallis test with significance level of P<0.05. Results: There was no significance in expression of mir-133a، Runx2 and PPARγ in 5 groups. Conclusion: Investigating cellular changes in bone after such exercises requires longer periods of exercise that should be investigated in future research..

کلیدواژه‌ها English

bone metabolism
femor bone
mir-133a
RUNX2
endurance and resistance training
PPARγ
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