اثر کوتاه‌مدت مکمل‌یاری عصارۀ دانۀ انگور سیاه بر ظرفیت آنتی‌اکسیدانی دختران غیر ورزشکار پس از یک جلسه فعالیت هوازی درمانده ساز

نویسندگان
شیما طاهرخانی 1
حمید محبی 1
حمید اراضی 1
ریحانه سریری 2
1 گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران
2 گروه بیوشیمی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران
چکیده
مقدمه

هدف این پژوهش، بررسی تأثیر مکمل‌یاری کوتاه‌مدت عصارۀ دانۀ انگور سیاه بر فعالیت آنزیم‌های آنتی‌اکسیدانی پس از یک جلسه فعالیت هوازی حاد در دختران غیر ورزشکار بود.

مواد و روش‌‌ها

16 دانشجوی دختر غیر ورزشکار به ‌شکل تصادفی به 2 گروه 8 نفری عصارۀ دانۀ انگور سیاه (مصرف 3 کپسول 100 میلی‌گرمی در روز به مدت 4 هفته) و دارونما (مصرف 3 کپسول 100 میلی‌گرمی مالتودکسترین در روز به مدت 4 هفته) تقسیم شدند. نمونه خونی در سه مرحله قبل و پس از 4 هفته مکمل‌یاری و بلافاصله پس از فعالیت هوازی بروس گرفته شد. داده‌ها از طریق آزمون‌ تحلیل واریانس با اندازه‌گیری مکرر در سطح معنی‌داری 0/05=α بررسی شدند.

یافته‌‌ها

نتایج نشان داد فعالیت آنزیم‌های سوپر اکسید دیسموتاز (SOD)، گلوتاتیون پراکسیداز (GPX) و پراکسیداز (POD) بین 2 گروه در حالت پایه و پس از مکمل‌یاری، تفاوت معنی‌داری نداشت. فعالیت آنزیم کاتالاز (CAT) پس از مکمل‌یاری بین 2 گروه معنی‌دار بود (0/05>P). فعالیت آنزیم‌های آنتی‌اکسیدانی در گروه مداخله در حالت پایه نسبت به پس از مکمل‌یاری و پس از فعالیت به‌ شکل معنی‌داری کمتر بود (0/05>P). میانگین فعالیت این آنزیم‌ها پس از فعالیت به‌‌طور معنی‌داری نسبت به پس از مکمل‌یاری در گروه مداخله بیشتر بود (0/05>P). در گروه دارونما در زمان پس از فعالیت نسبت به حالت پایه و پس از مکمل‌یاری به‌طور معنی‌داری بیشتر بود (0/05>P).

نتیجه‌‌گیری

بر اساس نتایج این تحقیق مکمل عصارۀ دانۀ انگور سیاه احتمالا افزایش فعالیت آنزیم‌های آنتی‌اکسیدانی پس از فعالیت حاد در دختران غیر ورزشکار را به همراه داشته باشد.
کلیدواژه‌ها
فشار اکسایشی
فعالیت هوازی درمانده ساز
عصارۀ دانۀ انگور

عنوان مقاله English

Short-term effects of grape seed extract supplementation on the antioxidant capacity of non-athlete girls following an exhaustive aerobic exercise

نویسندگان English

Shima Taherkhani 1
Hamid Mohebbi 1
Hamid Arazi 1
Reyhaneh Sariri 2
1 Department of Exercise Physiology, Faculty of Sport Sciences, University of Guilan, Rasht, Iran
2 Department of Biochemistry, Faculty of Basic Sciences, University of Guilan, Rasht, Iran
چکیده English

Introduction

The aim of the present study was to investigate the Short-term effect of grape seed extract (GSE) supplementation on the antioxidant capacity of non-athlete girls following an exhaustive aerobic exercise.

Materials and Methods

Sixteen non-athletes female students were randomly assigned into 2 groups of grape seed extract (three capsules of 100 mg per day for 4 weeks) and placebo (three capsules of maltodextrin 100 mg per day for 4 weeks). The first blood sampling was done from the brachial vein to measure the blood levels of the antioxidant enzymes mentioned; the second blood sampling was done after the 4-week period, Subjects then performed the Bruce aerobic exhaustive test protocol then, immediately after the finish of this test, the third blood sampling was done.

Results

The results showed that in terms of the activity of SOD, GPX and POD enzymes, in baseline and post-supplementation, there was no significant difference between the two supplement and placebo groups. The activity of CAT enzyme after the supplementation period was significant between the two groups (p<0.05). The activity antioxidant enzymes in the GSE group in the baseline was significantly lower than after the supplementation period and after the activity (p<0.05). The mean of these enzymes after exercise was significantly higher than supplementation (p<0.05). Were significantly higher in the placebo group after the activity than the baseline and after supplementation (p<0.05).

Conclusion

According to the results, supplementation of GSE improved the antioxidant enzymes mentioned in non-athlete girls after the aerobic exhaustive exercise.

کلیدواژه‌ها English

Oxidative stress
aerobic exhaustive exercise
grape seed extract
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