اثر 8 هفته تمرین تداومی به همراه مصرف مکمل رزوراترول بر نشانگران ضد اکسایشی(SOD و GPX) و نشانگر اکسایشی (MDA) بافت هپاتوسیتی در رت های نر دیابتی شده با استرپتوزوتوسین

نویسندگان
1 گروه فیزیولوژی ورزش، واحد کرمانشاه، دانشگاه آزاد اسلامی، کرمانشاه، ایران
2 گروه فیژیولوژی ورزش، واحد کرمانشاه، دانشگاه آزاد اسلامی ،کرمانشاه، ایران
3 گروه فیزیولوژی ورزش، واحد آبادان، دانشگاه آزاد اسلامی، آبادان، ایران
4 گروه فیزیولوژی ورزش، دانشکده تربیت بدنی، واحد تهران مرکز، دانشگاه آزاد اسلامی ، تهران، ایران
5 گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
چکیده
مقدمه

دیابت قندی یک بیماری مزمن جدی وپیچیده است وعاملی عمده دربوجود آمدن مشکلات سلامتی در سراسرجهان است. هدف این مطالعه تأثیر همزمان مکمل رزوراترول و تمرین تداومی برنشانگران ضد اکسایشی(SODوGPX)و نشانگراکسایشی (MDA) هپاتوسیتی در رت های نر نژاد ویستاردیابتی شده با استرپتوزوتوسین بود.

مواد و روش­ها

60 سر موش صحرایی نژاد ویستار شامل گروه های کنترل سالم، دیابتی، دیابتی سالین، مکمل رزوراترول، تمرین تداومی و مکمل رزوراترول+تمرین تداومی بررسی شدند. ارزیابی نشانگران (SOD وGPX) و (MDA) بوسیله ی کیت های الایزا انجام شد. برای تحلیل داده ها از آزمون تحلیل یک راهه واریانس و آزمون تعقیبی توکی استفاده شد، سطح معناداری 05/0 و نرم افزار SPSS 23 مورد استفاده قرار گرفت.

یافته­ها

میانگین غلظت SOD و GPX در گروه های بیمار دیابتی و دیابتی -سالین در مقایسه با سایر گروه ها به طور معنی داری کمتر (P≥0/001) و غلظت MDA در گروه های بیمار دیابتی و دیابتی سالین در مقایسه با سایر گروه ها به طور معنی داری بیشتر بوده است( .(P ≥0/001تمرین تداومی و تجویز رزوراتول به تنهایی و یا به ویژه مداخله رزوراترول همراه با ورزش تداومی سبب افزایش معنی داری در میانگین غلظت SOD و غلظت GPX ( (P ≥0/001 و کاهش معنی دار در میانگین غلظت MDAگردید ( (P ≥0/001.

نتیجه­گیری

استفاده از شیوه های درمانی غیردارویی تمرین ورزشی، مکمل رزوراترول و به ویژه ترکیبی از این دو می تواند در افزایش نشانگرهای ضد اکسایشی و کاهش نشانگرهای اکسایشی ناشی از بیماری دیابت هپاتوسیتی موثر باشد.
کلیدواژه‌ها

عنوان مقاله English

Effect of 8 week continuous training with resveratrol supplementation on anti-oxidative markers (SOD and GPX) and oxidative marker (MDA) of hepatocyte tissue in streptozotocin-diabetic male rats

نویسندگان English

Hamidreza Negharestani 1
Sedigheh Hosseinpour delavar 2
Masoumeh Azizi 3
Mohammad Ali Azarbaijani 4
parvin farzanegi 5
1 Department of Physiology of Sport, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
2 Department of Physiology of Sport, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
3 Department of Physical Education, Abadan Branch, Islamic Azad University, Abadan, Iran
4 Department of Physiology of Sport, Faculty of Physical Education, Tehran University Center, Islamic Azad University,Tehran, Iran
5 Department of Physiology, Sari Branch, Islamic Azad University, Sari, Iran
چکیده English

Introduction

Diabetes mellitus is a serious and complex chronic disease and is a major contributor to the health problems around the world. The purpose of this study was to compare the simultaneous effect of resveratrol supplementation and continuous training on anti-oxidative markers (SOD and GPX) and hepatocytic oxidative marker (MDA) in streptozotocin-diabetic male Wistar rats.

Materials and Methods

60 wistar rats including healthy control groups, diabetic, diabetic saline, resveratrol supplement, continuation training and complementary resveratrol + continuation training were evaluated. (SOD and GPX) and (MDA) were evaluated by ELISA kit. One-way analysis of variance and Tukey's post hoc test were used for data analysis. The significance level was considered 0.05 and SPSS 23 software was used.

Results

The mean concentration of SOD and GPX in diabetic and diabetic saline patients compared to other groups was significantly lower (P≥0/001) and MDA concentration in diabetic and diabetic saline patients compared to other groups was significantly higher (P ≥ 0/001). continuous training and resveratrol alone or in particular resveratrol intervention with continuous training significantly increased the mean concentration of SOD and GPX concentration (P≥0/001)and A significant decrease was observed in the mean concentration of MDA (P≥0/001).

Conclusion

The use of non-pharmacological exercise therapy, resveratrol supplementation, and in particular the combination of these two, can be effective in increasing the antioxidant markers and reducing oxidative markers due to hepatitis Diabetes.

کلیدواژه‌ها English

Diabetes Mellitus
Resveratrol
continuous training
SOD
GPX and MDA
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